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Isolation and Antimicrobial Activity of Lactic Acid Bacteria against Streptococcus Mutans

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dc.contributor.authorRahman N.Z.A.en_US
dc.contributor.authorHanafiah R.M.en_US
dc.contributor.authorGhafar S.A.A.en_US
dc.contributor.authorAbdullah N.en_US
dc.contributor.authorAzman N.N.en_US
dc.date.accessioned2024-05-29T02:02:40Z
dc.date.available2024-05-29T02:02:40Z
dc.date.issued2020
dc.descriptionJournal of International Dental and Medical Research ISSN1309-100X Volume ∙13∙ Number∙2∙ 2020en_US
dc.description.abstractThe aim of this study was to isolate lactic acid bacteria (LAB) from fermented food, to determine its antibacterial activity, and to identify the LAB in order to select candidate probiotic strains for preventing caries. The probiotic strains were isolated from eight fermented food which are tapai ubi, tapai pulut, rebung, tauchu, kimchi, cincalok, tempeh and tempoyak, taucu. Ten-fold serial dilutions of the fermented food samples were made in sterile peptone water before plating on de Man Rogosa and Sharpe (MRS) agar. The pure cultures were then randomly picked and biochemically identified. Then, the antibacterial activity of LAB against Streptococcus mutans was assessed by using the disk diffusion method. A total of 120 LAB were isolated from eight different fermented foods. The morphologies of the isolates were circular, convex, dull opaque white or translucent white. Gram staining identification showed that the isolates were Gram-positive rods. Of the 120 LAB isolates, five strains displayed moderate to strong antibacterial activity against S. mutans with the inhibition zones ranging from 7-12 mm. The antibacterial activity demonstrated from LAB isolated from fermented foods suggests that the isolates from fermented foods possess antibacterial properties against a pathogen which responsible for causing dental caries. These strains are currently investigated in depth to assess whether they can be fully characterized as probiotics. 2020en_US
dc.identifier.epage421
dc.identifier.issn1309100X
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85096475100
dc.identifier.spage417
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85096475100&partnerID=40&md5=40413dbdb2dc1e7cb99a6d90677ce8f0
dc.identifier.urihttp://www.jidmr.com
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10224
dc.identifier.volume13
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherUniversity of Dicleen_US
dc.relation.ispartofJournal of International Dental and Medical Researchen_US
dc.sourceScopus
dc.subjectdental cariesen_US
dc.subjectKeywords: Probioticsen_US
dc.subjectlactic acid bacteriaen_US
dc.subjectoral health.en_US
dc.subjectStreptococcus mutansen_US
dc.titleIsolation and Antimicrobial Activity of Lactic Acid Bacteria against Streptococcus Mutansen_US
dc.typeArticleen_US
dspace.entity.typePublication

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