Publication:
The use of Lates calcarifer as a biomarker for heavy metals detection

Simple item page
dc.citedby11
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Malaysia Perlis (UniMAP)
dc.contributor.authorHayat N.M.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorSabullah M.K.en_US
dc.contributor.authorShukor M.Y.en_US
dc.contributor.authorSyed M.A.en_US
dc.contributor.authorKhalid A.en_US
dc.contributor.authorDahalan F.A.en_US
dc.contributor.authorAhmad S.A.en_US
dc.date.accessioned2024-05-28T08:47:07Z
dc.date.available2024-05-28T08:47:07Z
dc.date.issued2016
dc.description.abstractFish are ubiquitous organisms that have many features that designate their potential as a biomarker of heavy metals pollution. Thus, an investigation was done to detect the effect of heavy metals on cholinesterase (ChE) activity from Lates calcarifer organs which were gill and muscle. Ammonium sulphate precipitation was performed along with ion exchange chromatography to purify the enzyme. In the substrate specificity study, ChE from L. calcarifer gills was capable of breaking down acetylthiocholine iodide (ATC) at a faster rate compared to the other two synthetic substrates, which are butyrylthiocholine iodide (BTC) and propionylthiocholine iodide (PTC). In contrast, the muscle ChE has a higher affinity towards PTC. The maximum activity of ChE observed at the temperature ranging from 20 to 30��C in Tris�HCl buffer pH 8. ChE from the two organs of L. calcarifer showed an inhibitive reaction towards heavy metals, but with different effects. ATC from gills showed 50�% inhibition by Cu, Hg and Pb, while PTC from muscle showed 50�% inhibition by Pb. The variation of inhibitory effect that was shown by ChE from L. calcarifer organs can be further studied in designing a biosensor kit that is sensitive towards heavy metal. � 2016, Accademia Nazionale dei Lincei.
dc.description.natureFinalen_US
dc.identifier.doi10.1007/s12210-015-0501-7
dc.identifier.epage472
dc.identifier.issn20374631
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84955268265
dc.identifier.spage463
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84955268265&doi=10.1007%2fs12210-015-0501-7&partnerID=40&md5=13cf74b9787d51b4be61f7ca2f841b05
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9452
dc.identifier.volume27
dc.languageEnglish
dc.language.isoen_US
dc.publisherSpringer-Verlag Italia s.r.l.en_US
dc.relation.ispartofRendiconti Lincei
dc.sourceScopus
dc.subjectBiomarkeren_US
dc.subjectBiosensoren_US
dc.subjectCholinesteraseen_US
dc.subjectHeavy metalsen_US
dc.subjectBiosensorsen_US
dc.subjectChromatographic analysisen_US
dc.subjectHeavy metalsen_US
dc.subjectIon chromatographyen_US
dc.subjectIon exchangeen_US
dc.subjectMuscleen_US
dc.subjectSubstratesen_US
dc.subjectSulfur compoundsen_US
dc.subjectAmmonium sulphateen_US
dc.subjectCholinesteraseen_US
dc.subjectHeavy metals detectionen_US
dc.subjectHeavy metals pollutionen_US
dc.subjectInhibitory effecten_US
dc.subjectIon exchange chromatographyen_US
dc.subjectSubstrate specificityen_US
dc.subjectSynthetic substratesen_US
dc.titleThe use of Lates calcarifer as a biomarker for heavy metals detection
dc.typeArticleen_US
dspace.entity.typePublication

Files

Collections

Scopus