Publication:
Exposure To High-fat Style Diet Induced Renal And Liver Structural Changes, Lipid Accumulation And Inflammation In Intact And Ovariectomized Female Rats

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dc.contributor.authorYamuna Sucedaramen_US
dc.contributor.authorEdward James Johnsen_US
dc.contributor.authorRuby Husainen_US
dc.contributor.authorMunavvar Abdul Sattaren_US
dc.contributor.authorMohammed H Abdullaen_US
dc.contributor.authorGiribabu Nellien_US
dc.contributor.authorNur Syahrina Binti Rahimen_US
dc.contributor.authorManizheh Khalilpourfarshbafien_US
dc.contributor.authorNor Azizan Abdullahen_US
dc.date.accessioned2024-05-28T04:22:22Z
dc.date.available2024-05-28T04:22:22Z
dc.date.issued2021
dc.date.submitted2022-2-16
dc.description.abstractPurpose: We hypothesized that low estrogen levels aggravate obesity-related complications. Diet-induced obesity can cause distinct pathologies, including impaired glucose tolerance, inflammation, and organ injury that leads to fatty liver and chronic kidney diseases. To test this hypothesis, ovariectomized (OVX) rats were fed a high-fat style diet (HFSD), and we examined structural changes and inflammatory response in the kidney and liver. Methods: Sprague-Dawley female rats were ovariectomized or sham-operated and divided into four groups: sham-operated rats fed a normal diet (ND); ovariectomized rats fed a normal diet (OVX-ND); sham-operated rats fed a HFSD; ovariectomized rats fed a high-fat style diet (OVX-HFSD). Mean blood pressure and fasting blood glucose were measured on weeks 0 and 10. The rats were sacrificed 10 weeks after initiation of ND or HFSD, the kidney and liver were harvested for histological, immunohistochemical and immunofluorescence studies. Results: HFSD-fed rats presented a significantly greater adiposity index compared to their ND counterparts. Liver index, fasting blood glucose and mean blood pressure was increased in OVX-HFSD rats compared to HFSD rats at study terminal. Histological and morphometric studies showed focal interstitial mononuclear cell infiltration in the kidney of HFSD rats with mesangial expansion being greater in the OVX-HFSD rats. Both HFSD fed groups showed increased expressions of renal inflammatory markers, namely TNF-alpha, IL-6 and MCP-1, and infiltrating M1 macrophages with some influence of ovarian hormonal status. HFSD-feeding also caused hepatocellular steatosis which was aggravated in ovariectomized rats fed the same diet. Furthermore, hepatocellular ballooning was observed only in the OVX-HFSD rats. Similarly, HFSD-fed rats showed increased expressions of the inflammatory markers and M1 macrophage infiltration in the liver; however, only IL-6 expression was magnified in the OVX-HFSD. Conclusion: Our data suggest that some of the structural changes and inflammatory response in the kidney and liver of rats fed a HFSD are exacerbated by ovariectomy.en_US
dc.identifier.citationSucedaram Y, Johns EJ, Husain R, Abdul Sattar M, H Abdulla M, Nelli G, Rahim NS, Khalilpourfarshbafi M, Abdullah NA. Exposure to High-Fat Style Diet Induced Renal and Liver Structural Changes, Lipid Accumulation and Inflammation in Intact and Ovariectomized Female Rats. J Inflamm Res. 2021;14:689-710 https://doi.org/10.2147/JIR.S299083en_US
dc.identifier.doi10.2147/JIR.S299083
dc.identifier.epage710
dc.identifier.issn1178-7031
dc.identifier.issue1
dc.identifier.other588-46
dc.identifier.spage689
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5414
dc.identifier.volume14
dc.language.isoenen_US
dc.publisherDove Pressen_US
dc.relation.ispartofJournal of Inflammation Researchen_US
dc.subjectobesity, ovariectomy, macrophage infiltration, mesangial expansion, hepatic steatosisen_US
dc.titleExposure To High-fat Style Diet Induced Renal And Liver Structural Changes, Lipid Accumulation And Inflammation In Intact And Ovariectomized Female Ratsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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