Publication:
Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil

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dc.contributor.authorDahalan, FAen_US
dc.contributor.authorYunus, Ien_US
dc.contributor.authorJohari, WLWen_US
dc.contributor.authorShukor, MYen_US
dc.contributor.authorHalmi, MIEen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSyed, MAen_US
dc.date.accessioned2024-05-29T02:56:12Z
dc.date.available2024-05-29T02:56:12Z
dc.date.issued2014
dc.description.abstractA diesel-degrading bacterium was isolated from a diesel-contaminated site in Selangor, Malaysia. The isolate was tentatively identified as Acinetobacter sp. strain DRY12 based on partial 16S rDNA molecular phylogeny and Biolog (R) GN microplate panels and Microlog (R) database. Optimum growth occurred from 3 to 5% diesel and the strain was able to tolerate as high as 8% diesel. The optimal pH that supported growth of the bacterium was between pH 7.5 to 8.0. The isolate exhibited optimal growth in between 30 and 35 degrees C. The best nitrogen source was potassium nitrate (between 0.6 and 0.9% (w/v)) followed by ammonium chloride, sodium nitrite and ammonium sulphate in descending order. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 10 days of incubation. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibiting growth with a correlation coefficient value of 0.97. The maximum growth rate- p(max) was 0.039 hr(-1) while the saturation constant or half velocity constant Ks and inhibition constant Ki, were 0.387% and 4.46%, respectively. MATH assays showed that 75% of the bacterium was found in the hexadecane phase indicating that the bacterium was hydrophobic. The characteristics of this bacterium make it useful for bioremediation works in the Tropics.
dc.identifier.epage406
dc.identifier.issn0254-8704
dc.identifier.issue2
dc.identifier.scopusWOS:000332500000016
dc.identifier.spage399
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11572
dc.identifier.volume35
dc.languageEnglish
dc.language.isoen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.ispartofJournal Of Environmental Biology
dc.sourceWeb Of Science (ISI)
dc.subjectIsolationen_US
dc.subjectCharacterizationen_US
dc.subjectDiesel-degradingen_US
dc.subjectAcinetobacter sp.en_US
dc.subjectHaldaneen_US
dc.titleGrowth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
dc.typeArticleen_US
dspace.entity.typePublication

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