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Isolation, identification, cultivation and determination of antimicrobial β-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662

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dc.contributor.authorRahayu Binti Ahmaden_US
dc.contributor.authorSrivani Sellathoroeen_US
dc.contributor.authorEhwan Bin Ngadien_US
dc.contributor.authorTengku Shafazila Bt Tengku Saharuddinen_US
dc.contributor.authorIffah Izzati Zakariaen_US
dc.contributor.authorSuguna Selvakumaranen_US
dc.contributor.authorWan Abd Al Qadr Imad Wan-Mohtaren_US
dc.date.accessioned2024-05-27T14:38:23Z
dc.date.available2024-05-27T14:38:23Z
dc.date.issued2021
dc.date.submitted2021-11-1
dc.description.abstractDuring the monsoon season, a wild-termite mushroom Termitomyces heimii RFES 230662 (THR2) was successfully isolated from Negeri Sembilan, Malaysia. The mushroom was morphologically labelled based on its stipe, pileus, and the budding mycelia from the termites nest. Genetic component of THR2 (300 bp) was sequenced and found to be 99% identical to Termitomyces sp. strain. The evolutionary distance (Knuc) of the isolate justified THR2 as T. heimii species. Two THR2 extracts were prepared from fruiting body (FB) and mycelial biomass (MB) for antimicrobial responses. THR2 was cultivated in a submerged-fermentation (SF) producing 8.55 g/L of MB, 0.80 g/L of endopolysaccharide (ENS) and 1.44 g/L of exopolysaccharide (EPS), respectively. For THR2-FB, two ENS extracts were obtained using hot (3.20 g/L) and cold-water (1.36 g/L) treatments. FTIR spectra analysis verified all polysaccharide as β-glucan when compared to laminarin standard. Those β-glucan possess antibacterial properties with highest response shown by ß-glucan-FB compared to ß-glucan-MB extracts. These findings serve as the blueprint for the production of β-glucan from a rare termite mushroom.en_US
dc.identifier.doi10.1016/j.bcab.2021.102187
dc.identifier.epage187
dc.identifier.issn1878-8181
dc.identifier.other2282-14
dc.identifier.spage102
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/3510
dc.identifier.volume37
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofBiocatalysis and Agriculture Biotechnologyen_US
dc.subjectExopolysaccharide; Medicinal mushroom; Mushroom cultivation; Termite; Termitomyces heimiien_US
dc.titleIsolation, identification, cultivation and determination of antimicrobial β-glucan from a wild-termite mushroom Termitomyces heimii RFES 230662en_US
dc.typeArticleen_US
dspace.entity.typePublication

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