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Sensitivity of acanthamoeba cyst to antimicrobial agents

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dc.citedby1
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorNoradilah S.A.en_US
dc.contributor.authorMohamed Kamel A.G.en_US
dc.contributor.authorAnisah N.en_US
dc.contributor.authorNoraina A.R.en_US
dc.contributor.authorYusof S.en_US
dc.date.accessioned2024-05-28T08:30:56Z
dc.date.available2024-05-28T08:30:56Z
dc.date.issued2010
dc.description.abstractIntroduction: Acanthamoeba is an ubiquitous free-living protozoa which causes serious ocular problems. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers. The disease can cause loss of vision and blindness if not treated properly. The objective of this research is to study the sensitivity of six Acanthamoeba spp. isolates, of which three were from the clinical isolates (HKL 95, HTH 40 and HS 6) and the remaining three from environmental isolates (TTT 9, TL 3 and SMAL 8) to antimicrobial agents. Methods: The antimicrobial agents chosen for this purpose were polyhexamethylene biguanide (PHMB) and Chlorhexidine. Serial dilutions were perfomed forpolyhexamemylene biguanide and Chlorhexidine. Cyst suspensions from the chosen isolates were exposed to PHMB and Chlorhexidine respectively. After 48 hours incubation time at 30�C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30�C and examined daily until day 14 to detect the presence of Acanthamoeba trophozoites under the inverted microscope. The presence of trophozoites indicated me ineffectiveness of the antimicrobial agents. Results: Both of the antimicrobial agents tested were found to be effective against Acanthamoeba cysts from all die test strains. Polyhexamethylene biguanide gave a minimum cysticidal concentration (MCC) mean value of 2.848 ug/mL while Chlorhexidine showed MCC mean value at a concentration of 3.988 ?g/mL. Contusion: It can be concluded that me Acanthamoeba cysts were sensitive to polyhexamethylene biguanide and Chlorhexidine.en_US
dc.description.natureFinalen_US
dc.identifier.epage117
dc.identifier.issn16758544
dc.identifier.issue1
dc.identifier.scopus2-s2.0-77952601526
dc.identifier.spage111
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77952601526&partnerID=40&md5=77325aba58b8a02b017c16a3629d1006
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8966
dc.identifier.volume6
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofMalaysian Journal of Medicine and Health Sciencesen_US
dc.sourceScopus
dc.subjectAcanthamoebaen_US
dc.subjectChlorhexidineen_US
dc.subjectPolyhexamethylene biguanideen_US
dc.subjectSensitivityen_US
dc.titleSensitivity of acanthamoeba cyst to antimicrobial agentsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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