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The improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)

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dc.contributor.authorZulfahmi, Sen_US
dc.contributor.authorYazan, LSen_US
dc.contributor.authorIthnin, Hen_US
dc.contributor.authorArmania, Nen_US
dc.date.accessioned2024-05-29T03:26:14Z
dc.date.available2024-05-29T03:26:14Z
dc.date.issued2013
dc.description.abstractCervical cancer is the most common gynecological cancer and one of the major causes of female cancer-related death worldwide particularly in developing countries. Thus far, there are a few in vivo models have been developed in investigating this type of cancer. In this study, we induced cervical cancer in Balb/c mice by exploiting the carcinogenic property of diestylstilbestrol (DES). The Balb/c pregnant mice were given subcutaneous (SC) injection of 67 mu g/kg body weight of DES on GD 13, and the mice gave birth approximately at gestation day 19-22. Female offspring were reared and the body weight was recorded once weekly. The female offspring were sacrificed at age of 5 months. Upon termination, blood was collected in a plain tube via cardiac puncture and the reproductive tracts were collected and weighed. The reproductive tract sections were stained using H&E for observation of pathological changes. The progression of disease state was monitored by measuring the level of serum interleukin (IL-6) using the Mouse IL-6 ELISA Assay Kit (BD OptEIA(TM), USA). All parameters were compared with Not-induced group. The outcome of this study demonstrated a significant difference in body weight gain, reproductive organ weight, diameter, of cervix and the level of serum IL-6 in the Induced group as compared to the Not-induced group (P<0.05). Histopathological findings revealed the presence of adenosis only in the Induced group. It shows that DES could be employed as an agent to induce cervical carcinogenesis in animal model. In addition to that, new potential anti-cancer agents from various sources could be further evaluated using this technique. (C) 2013 Published by Elsevier GmbH.
dc.identifier.doi10.1016/j.etp.2013.04.004
dc.identifier.epage1089
dc.identifier.issn0940-2993
dc.identifier.issue7-Aug
dc.identifier.scopusWOS:000327686300016
dc.identifier.spage1083
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12088
dc.identifier.volume65
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofExperimental And Toxicologic Pathology
dc.sourceWeb Of Science (ISI)
dc.subjectIn vivo modelen_US
dc.subjectBalb/c miceen_US
dc.subjectDiethylstilbestrolen_US
dc.subjectCarcinogenesisen_US
dc.subjectAdenosisen_US
dc.subjectInterleukin-6en_US
dc.titleThe improvement of in vivo model (Balb/c mice) for cervical carcinogenesis using diethylstilbestrol (DES)
dc.typeArticleen_US
dspace.entity.typePublication

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