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Recombinase Polymerase Amplification and Their Application in Phytopathogen Detection

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dc.contributor.authorWan Hawa Najibah Wan Rasnien_US
dc.contributor.authorNazariyah Yahayaen_US
dc.contributor.authorMaryam Mohamed Rehanen_US
dc.date.accessioned2024-05-30T02:08:56Z
dc.date.available2024-05-30T02:08:56Z
dc.date.issued2022
dc.descriptionVol. 8 No. 2 (2022)en_US
dc.description.abstractDNA identification method is indispensable for the detection of a plant pathogen. However, established techniques, though reliable, requires advanced equipment, and their application outside specialized laboratories is limited. Along with the advancement of molecular techniques, several isothermal amplification methods, including Recombinase Polymerase Amplification (RPA), has been developed in this study. In fact, RPA is a rapid and sensitive amplification method, operating optimally at 37-42 degree celcius for 15 to 30 minutes with minimal sample preparation, and can amplify as low as 1-10 target copies. Furthermore, RPA has been a favourable method for the detection of plant pathogens due to its advantageous parameters. This review presents the current knowledge of RPA and its application in plant pathogen detection.en_US
dc.identifier.citationWan Rasni, W. H. N., Yahaya, N., & Mohamed Rehan, M. (2022). Recombinase Polymerase Amplification and Their Application in Phytopathogen Detection. Malaysian Journal of Science Health & Technology, 8(2), 14–24. https://doi.org/10.33102/2022254en_US
dc.identifier.doi10.33102/2022254
dc.identifier.epage24
dc.identifier.issn2601-0003
dc.identifier.issue2
dc.identifier.spage14
dc.identifier.urihttps://mjosht.usim.edu.my/index.php/mjosht/article/view/254
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/15565
dc.identifier.volume8
dc.language.isoenen_US
dc.publisherUSIM Pressen_US
dc.relation.ispartofMalaysian Journal of Science, Health & Technology (MJoSHT)en_US
dc.subjectisothermal amplification, recombinase polymerase amplification, plant pathogens, diagnosticen_US
dc.titleRecombinase Polymerase Amplification and Their Application in Phytopathogen Detectionen_US
dc.typeArticleen_US
dspace.entity.typePublication

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