Publication:
Antibacterial and biofilm inhibition activities of Melastoma malabathricum stem bark extract against Streptococcus mutans

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dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorWan Syaidatul Aqmaen_US
dc.contributor.authorWan Ahmad Yaacoben_US
dc.contributor.authorZulfahmi Saiden_US
dc.contributor.authorNazlina Ibrahimen_US
dc.date.accessioned2024-05-29T01:57:21Z
dc.date.available2024-05-29T01:57:21Z
dc.date.issued2015
dc.descriptionMalaysian Journal of Microbiology, Vol 11(2) Special Issue 2015, pp. 199-206en_US
dc.description.abstractAim: This study was to determine the antibacterial activity of Melastoma malabathricum stem bark acetone extract (MMSBAE) against Streptococcus mutans. Methodology and results: Antibacterial activity of the extract was determined by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), biofilm formation, adherence inhibition, time kill studies and effect on S. mutans membrane integrity. MIC and MBC values of MMSBAE were 1.25 and 5 mg/mL, respectively. Time kill studies showed that reduction of colony forming unit in treated cells is 3 log10 after 10 h of treatment (p < 0.05). The extracts reduced 50% biofilm and adherence activity of S. mutans at 1.88 mg/mL. The effect on S. mutans membrane integrity after exposure to MMSBAE for 90 and 120 min was determined by measuring leakage of cell content at 2 different wavelengths of 260 nm and 280 nm. In leakage assay, the percentage of absorbance (260 nm) in treated cell material showed 57% at 90 min and 60% at 120 min which is higher than negative control (<20%) but less than positive control (100%). The percentage absorbance of treated cell material (280 nm) was 61% at 90 min and 63% at 120 min. Identification of compound in MMSBAE was done by gas chromatography mass spectrometry (GCMS). Ten compounds were identified in the MMSBAE with some of them important in antimicrobial activity such as ethyl ester, undecene, and gamma sitosterol. Conclusion, significance and impact of study: MMSBAE showed excellent bactericidal and antibacterial activities against S. mutans. The antibacterial mode of action of MMSBAE is suggested to be the disruption of the S. mutans membrane structure. The MMSBAE significantly inhibited biofilm and adherence activities of S. mutans in dose dependent manner (p < 0.05). MMSBAE has potential in the development of antibacterial agent with anti-biofilm and anti-adherence activities.en_US
dc.description.natureFinalen_US
dc.identifier.epage206
dc.identifier.issn22317538
dc.identifier.issueSpecialissue2
dc.identifier.scopus2-s2.0-84932602768
dc.identifier.spage199
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84932602768&partnerID=40&md5=c47c6ee662a28fc5ba840cdd335a03f2
dc.identifier.urihttps://mjm.usm.my/index.php?r=cms/entry/view&id=83&year=2015
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9919
dc.identifier.volume11
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.relation.ispartofMalaysian Journal of Microbiologyen_US
dc.sourceScopus
dc.subjectAntibacterialen_US
dc.subjectBiofilmen_US
dc.subjectMelastoma malabathricumen_US
dc.subjectStreptococcus mutansen_US
dc.titleAntibacterial and biofilm inhibition activities of Melastoma malabathricum stem bark extract against Streptococcus mutansen_US
dc.typeArticleen_US
dspace.entity.typePublication

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