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Endogenous and induced angiogenic characteristics of human chorion-derived stem cells

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dc.contributor.authorFariha, MMNen_US
dc.contributor.authorChua, KHen_US
dc.contributor.authorTan, GCen_US
dc.contributor.authorLim, YHen_US
dc.contributor.authorHayati, ARen_US
dc.date.accessioned2024-05-29T02:52:54Z
dc.date.available2024-05-29T02:52:54Z
dc.date.issued2012
dc.description.abstractCell-based therapy using stem cells has emerged as one of the pro-angiogenic methods to enhance blood vessel growth and sprouting in ischaemic conditions. This study investigated the endogenous and induced angiogenic characteristics of hCDSC (human chorion-derived stem cell) using QPCR (quantitative PCR) method, immunocytochemistry and fibrin-matrigel migration assay. The results showed that cultured hCDSC endogenously expressed angiogenic-endogenic-associated genes (VEGF, bFGF, PGF, HGF, Ang-1, PECAM-1, eNOS, Ve-cad, CD34, VEGFR-2 and vWF), with significant increase in mRNA levels of PGF, HGF, Ang-1, eNOS, VEGFR-2 and vWF following induction by bFGF (basic fibroblast growth factor) and VEGF (vascular endothelial growth factor). These enhanced angiogenic properties suggest that induced hCDSC provides a stronger angiogenic effect for the treatment of ischaemia. After angiogenic induction, hCDSC showed no reduction in the expression of the stemness genes, but had significantly higher levels of mRNA of Oct-4, Nanog (3), FZD9, ABCG-2 and BST-1. The induced cells were positive for PECAM-1 (platelet/endothelial cell adhesion molecule 1) and vWF (von Willebrand factor) with immunocytochemistry staining. hCDSC also showed endothelial migration behaviour when cultured in fibrin-matrigel construct and were capable of forming vessels in vivo after implanting into nude mice. These data suggest that hCDSC could be the cells of choice in the cell-based therapy for pro-angiogenic purpose.
dc.identifier.doi10.1042/CBI20120044
dc.identifier.epage1153
dc.identifier.issn1065-6995
dc.identifier.issue12
dc.identifier.scopusWOS:000311144500008
dc.identifier.spage1145
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11347
dc.identifier.volume36
dc.languageEnglish
dc.language.isoen_US
dc.publisherWileyen_US
dc.relation.ispartofCell Biology International
dc.sourceWeb Of Science (ISI)
dc.subjectangiogenesisen_US
dc.subjectcell-based therapyen_US
dc.subjectfetal stem cellsen_US
dc.subjectgrowth factorsen_US
dc.subjectischaemiaen_US
dc.subjectplacentaen_US
dc.titleEndogenous and induced angiogenic characteristics of human chorion-derived stem cells
dc.typeArticleen_US
dspace.entity.typePublication

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