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A large response range reflectometric urea biosensor made from silica-gel nanoparticles

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dc.citedby18
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsInstitute for Medical Research
dc.contributor.authorAlqasaimeh M.en_US
dc.contributor.authorHeng L.Y.en_US
dc.contributor.authorAhmad M.en_US
dc.contributor.authorSanthana Raj A.S.en_US
dc.contributor.authorLing T.L.en_US
dc.date.accessioned2024-05-28T08:32:48Z
dc.date.available2024-05-28T08:32:48Z
dc.date.issued2014
dc.description.abstractA new silica-gel nanospheres (SiO2NPs) composition was formulated, followed by biochemical surface functionalization to examine its potential in urea biosensor development. The SiO2NPs were basically synthesized based on sol-gel chemistry using a modified Stober method. The SiO2NPs surfaces were modified with amine (-NH2) functional groups for urease immobilization in the presence of glutaric acid (GA) cross-linker. The chromoionophore pH-sensitive dye ETH 5294 was physically adsorbed on the functionalized SiO2NPs as pH transducer. The immobilized urease determined urea concentration reflectometrically based on the colour change of the immobilized chromoionophore as a result of the enzymatic hydrolysis of urea. The pH changes on the biosensor due to the catalytic enzyme reaction of immobilized urease were found to correlate with the urea concentrations over a linear response range of 50-500 mM (R 2 = 0.96) with a detection limit of 10 mM urea. The biosensor response time was 9 min with reproducibility of less than 10% relative standard deviation (RSD). This optical urea biosensor did not show interferences by Na +, K +, Mg 2+ and NH4 + ions. The biosensor performance has been validated using urine samples in comparison with a non-enzymatic method based on the use of p-dimethylaminobenzaldehyde (DMAB) reagent and demonstrated a good correlation between the two different methods (R 2 = 0.996 and regression slope of 1.0307). The SiO2NPs-based reflectometric urea biosensor showed improved dynamic linear response range when compared to other nanoparticle-based optical urea biosensors.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.3390/s140713186
dc.identifier.epage13209
dc.identifier.issn14248220
dc.identifier.issue7
dc.identifier.pmid25054632
dc.identifier.scopus2-s2.0-84904696168
dc.identifier.spage13186
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84904696168&doi=10.3390%2fs140713186&partnerID=40&md5=b5e2ba999da324ac9b8542a1423c301c
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9035
dc.identifier.volume14
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofSensors (Switzerland)
dc.sourceScopus
dc.subjectLinear response rangeen_US
dc.subjectpH-sensitiveen_US
dc.subjectReflectometricen_US
dc.subjectSilica-gel nanospheresen_US
dc.subjectUrea biosensoren_US
dc.titleA large response range reflectometric urea biosensor made from silica-gel nanoparticlesen_US
dc.title.alternativeSensorsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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