Gas Chromatography-Mass Spectrometry Profiling of Extracted Compounds for Gum Disease Treatment: A Methodological Approach

The object of Tamarindus indica and Brucea javanica are investigated in this study as possible natural alternatives to allopathic medicines, focusing particularly on allergies and hyper-sensitivities towards synthetic chemicals in dental practices. Patient safety is a major concern given the adverse effects of drug allergies on care and results. The research sought to find out the therapeutic properties of these plants although there is need for more investigations into their efficacy. Extraction was done using acetone so as to obtain the extracts which exhibited several bioactive compounds. In rural areas, Tamarindus indica is often used for treating different diseases. Thereafter, the dry samples were ground into a fine powder, weighed accurately before placing them in a conical flask that contained hexane acetone solvent system at 4:1 ratio. The flask was then covered with an aluminum foil after being sealed overnight for 24 hours in order to allow complete extraction. The mixture was filtered separating the solid residue from solvent solution. This led to concentration of extracted compounds resulting into oil extract using rotary evaporator at 76°C and 264 atm. Solvent-to-sample weight ratio was 1:10; hence, ten milliliters per gram of sample were used during this process. This extraction method will be utilized for subsequent analysis using Gas Chromatography-Mass Spectrometry