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Effect of bacterial growth period on the sensitivity of the MTT assay for silver

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dc.contributor.authorHalmi, MIEen_US
dc.contributor.authorAhmad, Fen_US
dc.contributor.authorHashim, AKen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSyed, MAen_US
dc.contributor.authorShukor, MYen_US
dc.date.accessioned2024-05-29T03:25:42Z
dc.date.available2024-05-29T03:25:42Z
dc.date.issued2014
dc.description.abstractRespiratory activity inhibition by toxic compounds in bacteria and yeast has been used to detect toxic compounds in the environment. Often the age of culture contributes towards the sensitivity of detection. In the present work, the effect of growth period on the sensitivity of an inhibitive assay for heavy metals using bacterial respiratory assay system based on the reduction of the water soluble tetrazolium dye MU is reported. A silver-sensitive isolate was discovered to exhibit different sensitivities towards silver at different growth periods. An exponential decay model adequately described the inhibition due to silver. Analysis using ANOVA with post-hoc Tukey's test showed that the IC50 obtained by strain DRYS8 grown at the 12 hr-period in nutrient broth at 28 degrees C gave the lowest value compared to other growth periods. This study highlights the importance of taking into accounts growth conditions and age of culture in developing cellular-based bioassays.
dc.identifier.epage355
dc.identifier.issn0254-8704
dc.identifier.issue2
dc.identifier.scopusWOS:000332500000008
dc.identifier.spage353
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12010
dc.identifier.volume35
dc.languageEnglish
dc.language.isoen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.ispartofJournal Of Environmental Biology
dc.sourceWeb Of Science (ISI)
dc.subjectMTT assayen_US
dc.subjectBacteriumen_US
dc.subjectGrowth Perioden_US
dc.subjectSilveren_US
dc.titleEffect of bacterial growth period on the sensitivity of the MTT assay for silver
dc.typeArticleen_US
dspace.entity.typePublication

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