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Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells

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dc.Chemicals/CAS5' nucleotidase, 9027-73-0; collagenase, 37288-86-1, 39433-96-0; nestin, 146315-66-4; protein tyrosine phosphatase; receptor type tyrosine protein phosphatase C; von Willebrand factor, 109319-16-6
dc.FundingDetailsUniversiti Sains Islam Malaysia,�Islamic Science University Of Malaysia: PPP/USG-0216/FPSK/30/18016
dc.FundingDetailsThis research was funded by the Universiti Sains Islam Malaysia research grant (PPP/USG-0216/FPSK/30/18016).
dc.ManufacturersBecton Dickinson, Belgium
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsHospital Selayang
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.authorHayati A.-R.en_US
dc.contributor.authorAdzman-Sellymiahen_US
dc.contributor.authorChua K.-H.en_US
dc.contributor.authorAqilah R.-N.F.en_US
dc.contributor.authorTan G.-C.en_US
dc.contributor.authorFatimah S.-S.en_US
dc.contributor.authorLim Y.-H.en_US
dc.contributor.authorNur Syahrina Binti Rahimen_US
dc.contributor.authorFariha M.-M.N.en_US
dc.date.accessioned2024-05-28T08:27:37Z
dc.date.available2024-05-28T08:27:37Z
dc.date.issued2018
dc.description.abstractMesenchymal stem cells population has been successfully isolated from the matrix and characterised for its stemness properties. Endothelial cells from the vein of the umbilical cord which are usually termed as HUVEC are also of interest to researchers as it can be manipulated in vitro to study endothelial cells behaviour and vascular-associated diseases. Immunophenotyping for positive and negative markers of mesenchymal stem cells was performed on HUVEC and compared to the closest stem cells that were isolated from the umbilical cord matrix which is termed as HUCMSC in this study. The expressions of a set of stemness genes were also detected using qPCR. The results showed that HUVEC strongly expressed hematopoietic and mesenchymal stem cells markers (CD31, CD34, CD9, CD44, CD73 and CD90) while HUCMSC possess mesenchymal stem cells properties with strong expression of CD9, CD44, CD73 and CD90. Interestingly, the expression of stemness genes is detected higher in HUVEC compared to HUCMSC with possibilities of having neural properties as evidenced by the high expression of FGF4, FZD9, Nestin and SOX2. Thus, it is suggested that HUVEC exhibited some stem cells characteristics and could be further investigated for possible application in research as well as in future cells therapy. � 2018 Pharmascope Publications. All rights reserved.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.26452/ijrps.v9iSPL2.1741
dc.identifier.epage59
dc.identifier.issn9757538
dc.identifier.issueSpecial Issue 2
dc.identifier.scopus2-s2.0-85061549234
dc.identifier.spage51
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061549234&doi=10.26452%2fijrps.v9iSPL2.1741&partnerID=40&md5=aa96231b0168fced95071da9d9021e50
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8808
dc.identifier.volume9
dc.languageEnglish
dc.language.isoenen_US
dc.publisherJ. K. Welfare and Pharmascope Foundationen_US
dc.relation.ispartofInternational Journal of Research in Pharmaceutical Sciences
dc.sourceScopus
dc.subjectCell differentiationen_US
dc.subjectFlow cytometryen_US
dc.subjectRibonucleic acid (RNA)en_US
dc.subjectStem cellsen_US
dc.subjectTissue engineeringen_US
dc.titleComparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cellsen_US
dc.title.alternativeInt. J. Res. Pharm. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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