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Melastoma Malabathricum Stem Bark Acetone Extract As An Anti-bacterial Agent Against Streptococcus Mutans

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dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorNur Syahirah Salehuddinen_US
dc.contributor.authorWan Syaidatul Aqmaen_US
dc.contributor.authorNazlina Ibrahimen_US
dc.date.accessioned2024-05-28T04:22:15Z
dc.date.available2024-05-28T04:22:15Z
dc.date.issued2020
dc.date.submitted14/12/2020
dc.descriptionVolume: 11 No: 4 (page : 6986–6995)en_US
dc.description.abstractMelastoma malabathricum, also known as 'senduduk' in Malaysia, has been used as traditional medicine for diseases such as toothache, dysentery, haemorrhoids and stomachache. Therefore, the objective of this study is to investigate the biological activity of Melastoma malabathricum stem bark extracts (MMSBE) towards Streptococcus mutans. This investigation involved a few methods, which at first is the determination of minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). Next is by analyzing the time-kill curve, anti-biofilm activity, scanning electron and transmission electron microscopic analyses. Later, next-generation sequencing (NGS) was done to determine differential regulation genes of treated and non-treated S. mutans. Lastly, confirmation of differential regulation genes was done by RT-PCR analysis. As for the results, M. malabathricum stem bark acetone extract (MMSBAE) showed the greatest inhibition concentrations towards S. mutans, followed by M. malabathricum methanol extract (MMSBME). Values of MIC and MBC (MMSBAE) were 1.25 mg/mL and 5 mg/mL. Meanwhile, MIC and MBC values of MMSBME were 5 mg/mL and 40 mg/mL. MMSBAE was chosen to further analyze its anti-bacterial activity against S. mutans. Time kill curve analysis found that MMSBAE possessed bacteriostatic properties against S. mutans. Besides, SEM and TEM analyses revealed that there were some changes to S. mutans cell morphology after treated with MMSBAE while Next gene sequencing analysis revealed significant (p<0.05) gene expression with multiple targets by MMSBAE, which caused inhibition of S. mutans biofilm formation activity.en_US
dc.identifier.citationRohazila Mohamad Hanafiah, Siti Aisyah Abd Ghafar, Nur Syahirah Salehuddin, Wan Syaidatul Aqma, & Nazlina Ibrahim. (2020). Melastoma malabathricum stem bark acetone extract as an anti-bacterial agent against Streptococcus mutans. International Journal of Research in Pharmaceutical Sciences, 11(4), 6986–6995. Retrieved from https://ijrps.com/index.php/home/article/view/1087en_US
dc.identifier.epage6995
dc.identifier.issue4
dc.identifier.spage6986
dc.identifier.urihttps://ijrps.com/index.php/home/article/view/1087
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5405
dc.identifier.volume11
dc.language.isoen_USen_US
dc.publisherPharmascope Foundationen_US
dc.relation.ispartofInternational Journal of Research in Pharmaceutical Sciences (IJRPS)en_US
dc.subjectAnti Bacterial Agent, Melastoma malabathricum, Next Gene Sequencing, Streptococcus mutansen_US
dc.titleMelastoma Malabathricum Stem Bark Acetone Extract As An Anti-bacterial Agent Against Streptococcus Mutansen_US
dc.typeArticleen_US
dspace.entity.typePublication

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