Publication: Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development
dc.Chemicals/CAS | immunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6 | |
dc.FundingDetails | Universiti Sains Malaysia | |
dc.FundingDetails | This work was supported by Research University Cluster Grant Scheme (1001/PSKBP/8630015). EBO gratefully acknowledges the postdoctoral fellowship from USM during the course of this work. | |
dc.citedby | 7 | |
dc.contributor.affiliations | Chancellory Building | |
dc.contributor.affiliations | Universiti Sains Malaysia (USM) | |
dc.contributor.affiliations | Universiti Sains Islam Malaysia (USIM) | |
dc.contributor.author | Ong E.B.B. | en_US |
dc.contributor.author | Anthony A.A. | en_US |
dc.contributor.author | Ismail A. | en_US |
dc.contributor.author | Ismail A. | en_US |
dc.contributor.author | Lim T.S. | en_US |
dc.date.accessioned | 2024-05-28T08:29:51Z | |
dc.date.available | 2024-05-28T08:29:51Z | |
dc.date.issued | 2013 | |
dc.description.abstract | The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc. | en_US |
dc.description.nature | Final | en_US |
dc.identifier.CODEN | DMIDD | |
dc.identifier.doi | 10.1016/j.diagmicrobio.2013.05.010 | |
dc.identifier.epage | 89 | |
dc.identifier.issn | 7328893 | |
dc.identifier.issue | 1 | |
dc.identifier.pmid | 23790417 | |
dc.identifier.scopus | 2-s2.0-84881544694 | |
dc.identifier.spage | 87 | |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84881544694&doi=10.1016%2fj.diagmicrobio.2013.05.010&partnerID=40&md5=6c9793de23d716da35ad8743cd9e0f66 | |
dc.identifier.uri | https://oarep.usim.edu.my/handle/123456789/8921 | |
dc.identifier.volume | 77 | |
dc.language | English | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier Inc. | en_US |
dc.relation.ispartof | Diagnostic Microbiology and Infectious Disease | en_US |
dc.source | Scopus | |
dc.subject | Antigen | en_US |
dc.subject | HlyE | en_US |
dc.subject | Immunoblot, ELISA | en_US |
dc.subject | Salmonella | en_US |
dc.subject | Typhi | en_US |
dc.title | Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development | en_US |
dc.title.alternative | Diagn. Microbiol. Infect. Dis. | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication |
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