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Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development

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dc.Chemicals/CASimmunoglobulin G, 97794-27-9; immunoglobulin M, 9007-85-6
dc.FundingDetailsUniversiti Sains Malaysia
dc.FundingDetailsThis work was supported by Research University Cluster Grant Scheme (1001/PSKBP/8630015). EBO gratefully acknowledges the postdoctoral fellowship from USM during the course of this work.
dc.citedby7
dc.contributor.affiliationsChancellory Building
dc.contributor.affiliationsUniversiti Sains Malaysia (USM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorOng E.B.B.en_US
dc.contributor.authorAnthony A.A.en_US
dc.contributor.authorIsmail A.en_US
dc.contributor.authorIsmail A.en_US
dc.contributor.authorLim T.S.en_US
dc.date.accessioned2024-05-28T08:29:51Z
dc.date.available2024-05-28T08:29:51Z
dc.date.issued2013
dc.description.abstractThe hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation. � 2013 Elsevier Inc.en_US
dc.description.natureFinalen_US
dc.identifier.CODENDMIDD
dc.identifier.doi10.1016/j.diagmicrobio.2013.05.010
dc.identifier.epage89
dc.identifier.issn7328893
dc.identifier.issue1
dc.identifier.pmid23790417
dc.identifier.scopus2-s2.0-84881544694
dc.identifier.spage87
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84881544694&doi=10.1016%2fj.diagmicrobio.2013.05.010&partnerID=40&md5=6c9793de23d716da35ad8743cd9e0f66
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8921
dc.identifier.volume77
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherElsevier Inc.en_US
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_US
dc.sourceScopus
dc.subjectAntigenen_US
dc.subjectHlyEen_US
dc.subjectImmunoblot, ELISAen_US
dc.subjectSalmonellaen_US
dc.subjectTyphien_US
dc.titleCloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay developmenten_US
dc.title.alternativeDiagn. Microbiol. Infect. Dis.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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