Publication:
Evaluation on antibacterial activity of lactobacillus acidophilus strains isolated from honey

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dc.citedby16
dc.contributor.affiliationsFaculty of Science and Technology
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.authorAween M.M.en_US
dc.contributor.authorHassan Z.en_US
dc.contributor.authorMuhialdin B.J.en_US
dc.contributor.authorNoor H.M.en_US
dc.contributor.authorEljamel Y.A.en_US
dc.date.accessioned2024-05-29T02:05:19Z
dc.date.available2024-05-29T02:05:19Z
dc.date.issued2012
dc.description.abstractProblem statement: This study reports the isolation of lactic acid bacteria from 13 honey samples produced in Malaysia, Libya and Saudi Arabia and their antibacterial activity against three Gram negative pathogenic bacteria. Approach: A modified MRS agar with 0.8% CaCO3 and MRS with 1% glucose was found to facilitate isolation of LAB compared to MRS, tomato juice agar and modified tomato juice agar. 32 isolates were confirmed LAB by catalase test and Gram staining. Six isolates were screened for antibacterial activity and identified as strains of Lactobacillus acidophilus 1 by API CH50. Results: All the isolates showed very good inhibitory activity against target Gram negative bacteria as indicated by the diameter of inhibition zone: Salmonella Typhimurium (23-30 mm), Escherichia coli (7-18 mm) and Enterobacter aerogenes (10-18 mm) after 24 h incubation at 30�C. Supernatants of L. acidophilus 1 strains showed good antibacterial activity against all target bacteria. Heating the supernatants at 90 and 121�C for 1 h enhanced the antibacterial activity against all target bacteria except supernatants H006-A and H010-G against S. Typhimurium. Antibacterial activity of supernatants were maintained after pH adjustment to 3, but at pH5 supernatants H006-A, H008-D and H010-G lost the activity against S. Typhimurium and E. coli within 48 h of incubation while at pH 6 all supernatants lost activity except against E. aerogenes. Enzymes treatments of supernatants with RNase II and Proteinase K for 1 h inhibited all target bacteria except supernatants H008-D, H008-E and H006-A which were relatively sensitive to both enzymes against S. Typhimurium and E. coli. Conclusion/Recommendations: In conclusion, honey from different sources contains strains of L. acidophilus 1 that produced compounds with good antibacterial activity which may be responsible for the antibacterial properties of honey. � 2012 Science Publications.
dc.description.natureFinalen_US
dc.identifier.doi10.3844/ajassp.2012.807.817
dc.identifier.epage817
dc.identifier.issn15469239
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84858195925
dc.identifier.spage807
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84858195925&doi=10.3844%2fajassp.2012.807.817&partnerID=40&md5=9a41cf7e82efdb7b97c63401a85705d5
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10325
dc.identifier.volume9
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofAmerican Journal of Applied Sciences
dc.sourceScopus
dc.subjectAntibacterial activityen_US
dc.subjectAntibacterial propertiesen_US
dc.subjectDemonstrated antimicrobialen_US
dc.subjectIntensive care units (ICUs)en_US
dc.subjectLactic acid bacteria (LAB)en_US
dc.subjectMultiple antibiotic resistant (MAR)en_US
dc.titleEvaluation on antibacterial activity of lactobacillus acidophilus strains isolated from honeyen_US
dc.title.alternativeAm. J. Appl. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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