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Plagioneurin B, a potent isolated compound induces apoptotic signalling pathways and cell cycle arrest in ovarian cancer cells

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dc.Chemicals/CAScaspase 3, 169592-56-7; caspase 8; caspase 9, 180189-96-2; fluorescein isothiocyanate, 25168-13-2, 27072-45-3, 3326-32-7; lipocortin 5, 111237-10-6; protein bcl 2, 219306-68-0; survivin, 195263-98-0; Acetogenins; Antineoplastic Agents, Phytogenic; Caspase 8; Caspase 9; Plant Extracts
dc.FundingDetailsFL001E-13BIO
dc.FundingDetailsAcknowledgements We wish to acknowledge the Institute of Research Management and Monitory, University of Malaya under Flagship grant (FL001E-13BIO) for their financial support. We also dedicate this study to the late Prof. Datuk Dr. A. Hamid A. Hadi for his guidance and assistance in this study.
dc.citedby2
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversity of Malaya (UM)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.authorNordin N.en_US
dc.contributor.authorMajid N.A.en_US
dc.contributor.authorOthman R.en_US
dc.contributor.authorOmer F.A.A.en_US
dc.contributor.authorNasharuddin M.N.A.en_US
dc.contributor.authorHashim N.M.en_US
dc.date.accessioned2024-05-28T08:41:30Z
dc.date.available2024-05-28T08:41:30Z
dc.date.issued2018
dc.description.abstractPlagioneurin B belongs to acetogenin group has well-established class of compounds. Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). Its structure was also docked at several possible targets using Autodock tools software. Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC50 of 0.62��M. The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. The apoptosis event was triggered through the extrinsic and intrinsic pathways via activation of caspases 8 and 9, respectively. Stimulation of caspase 3 and the presence of DNA ladder suggested downstream apoptotic signalling were initiated. Further confirmation of apoptosis was conducted at the molecular levels where up-regulation in Bax, as well as down-regulation of Bcl-2, Hsp-70 and survivin were observed. Plagioneurin B was also seen to arrest CAOV-3 cells cycle at the G2/M phase. Docking simulation of plagioneurin B with CD95 demonstrated that the high binding affinity and hydrogen bonds formation may explain the capability of plagioneurin B to trigger apoptosis. This study is therefore importance in finding the effective compound that may offer an alternative drug for ovarian cancer treatment. � 2018, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.description.natureFinalen_US
dc.identifier.CODENAPOPF
dc.identifier.doi10.1007/s10495-018-1447-x
dc.identifier.epage169
dc.identifier.issn13608185
dc.identifier.issue2
dc.identifier.pmid29430581
dc.identifier.scopus2-s2.0-85041904621
dc.identifier.spage152
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85041904621&doi=10.1007%2fs10495-018-1447-x&partnerID=40&md5=f282e66f7aa0f410eb747d6b88adfee1
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9297
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherSpringer New York LLCen_US
dc.relation.ispartofApoptosis
dc.sourceScopus
dc.subjectAcetogeninen_US
dc.subjectApoptosisen_US
dc.subjectCAOV-3 cellsen_US
dc.subjectOvarian canceren_US
dc.subjectPathwaysen_US
dc.subjectPlagioneurin Ben_US
dc.titlePlagioneurin B, a potent isolated compound induces apoptotic signalling pathways and cell cycle arrest in ovarian cancer cellsen_US
dc.title.alternativeApoptosisen_US
dc.typeArticleen_US
dspace.entity.typePublication

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