Publication: Phytochemical analysis of Quercus infectoria galls extracts using FTIR, LC-MS and MS/MS analysis
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Date
2017
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Publisher
Research Journal of BioTechnology
Abstract
The present study was carried out to characterise and compare types of phytoconstituents present in crude and aqueous extract of Quercus infectoria (QI) using FTIR, LC-MS followed by confirming using MS/MS analysis. QI gall was purchased from local market and grinded to obtain the crude in powdered form. The aqueous extract produced by refluxing in water bath at 50°C for 24 hours was filtered and concentrated using rotary evaporator followed by freeze-drying. The crude and aqueous extract was scanned at wavelength 400 to 3400 nm using Tensor-II-FTIR spectrometer. LC-MS analysis utilised Agilent-1290-Infinity-system coupled to Agilent-6520-Accurate-Mass-Q-TOF mass-spectrometer with dual ESI. Detected constituents matched with METLIN_AM_PCDL-N-130328.cdb database. Compounds with >95 DB score were selected to be confirmed with MS/MS analysis. The FTIR spectrum confirmed presence of several similar functional groups in both crude and aqueous extract. LC-MS screening results indicated similar phytoconstituents present in both crude and aqueous QI (2.64% and 2.16%) respectively. MS/MS analysis confirmed presence of all targeted constituents in aqueous QI extract. In conclusion, LC-MS and MS/MS results show that all major constituents analysed were from polyphenolic derivatives primarily gallic acid and hydrolysable tannins.
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Keywords
FTIR,, LC-MS,, MS/MS,, Phytochemical,, Quercus infectoria (QI).
Citation
RESEARCH JOURNAL OF BIOTECHNOLOGY, 2017; 12 (12): 55