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Antibacterial Activity of Calcium Hydroxide and Zinc Oxide Combined with Several Solutions against Enterococcus faecalis Growth

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dc.contributor.authorSyatirah Najmi Binti Abdullah
dc.contributor.authorWan Nur Faizatulakmal Wan Ahmad Zaki
dc.contributor.authorSyarifah Musyirah Qistina Sayed Mansor
dc.contributor.authorAws Hashim Ali Al-Kadhim
dc.contributor.authorSiti Aisyah Abd Ghafar
dc.contributor.authorRohazila Mohamad Hanafiah
dc.date.accessioned2024-09-25T17:05:11Z
dc.date.available2024-09-25T17:05:11Z
dc.date.issued2024
dc.date.submitted2024-9-25
dc.descriptionEuropean Journal of General Dentistry
dc.description.abstractObjective This study aims to compare the antimicrobial activity of calcium hydroxide (CaOH) and zinc oxide (ZnO) when incorporated with other solutions such as 2% chlorhexidine (CHX), 2.5% sodium hypochlorite (NaOCl), 1% povidone-iodine (PVP-I), and sterilized distilled water (ddH2O) against Enterococcus faecalis. Materials and Methods The materials were prepared by mixing CaOH and ZnO with other solutions (CHX, PVP-I, NaOCl, and ddH2O) separately. The antibacterial activity of CaOH and ZnO mixtures against E. faecalis was done by using disk diffusion assay (DDA). Twofold serial dilutions of the mixtures were used against E. faecalis to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Biofilm inhibition of E. faecalis had been measured by using crystal violet assay. Statistical Analysis The quantitative data of this study had been analyzed by using two-way analysis of variance with software SPSS version 27. The result is considered as significant if the value of analysis is p-value less than 0.05. Results From the DDA results, the lowest zone of inhibition toward E. faecalis was CaOH-PVP-I (6.00 0.00 mm), while the highest zone of inhibition toward E. faecalis was CaOH-CHX (22.73 0.02 mm). Besides that, ZnO-PVP-I showed the lowest zone of inhibition (16.50 0.06 mm), while ZnO-CHX showed the highest zone of inhibition (18.30 0.08 mm) against E. faecalis. The MIC and MBC values of CaOH-CHX and ZnOCHX were 0.78 and 6.25 mg/mL, respectively. In biofilm assay, CaOH-CHX and ZnOCHX were reduced biofilm formation of E. Faecalis. Conclusion Both CaOH-CHX and ZnO-CHX showed the highest antimicrobial activities toward E. faecalis. CaOH and ZnO alone showed no antimicrobial activities against E. faecalis.
dc.identifier.citationSyatirah-Najmi Abdullah, Wan Nur Faizatulakmal Wan Ahmad Zaki, Syarifah Musyirah Qistina Sayed Mansor, Aws Hashim Ali Al-Kadhim, Siti Aisyah Abd Ghafar & Rohazila Mohamad Hanafiah (2024). Antibacterial Activity of Calcium Hydroxide and Zinc Oxide Combined with Several Solutions against Enterococcus faecalis Growth. European Journal of General Dentistry. https://doi.org/10.1055/s-0044-1786547
dc.identifier.doi10.1055/s-0044-1786547
dc.identifier.epage8
dc.identifier.issn2278-9626
dc.identifier.issue2024
dc.identifier.other1209-29
dc.identifier.spage1
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/22948
dc.identifier.urihttps://www.thieme-connect.com/products/ejournals/pdf/10.1055/s-0044-1786547.pdf
dc.identifier.volume2024
dc.language.isoen_US
dc.publisherThieme
dc.relation.ispartofEuropean Journal of General Dentistry
dc.relation.issn2320-4753
dc.relation.journalEuropean Journal of General Dentistry
dc.subjectcalcium hydroxide
dc.subjectzinc oxide
dc.subjectEnterococcus faecalis
dc.titleAntibacterial Activity of Calcium Hydroxide and Zinc Oxide Combined with Several Solutions against Enterococcus faecalis Growth
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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