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Molybdenum Reduction to Molybdenum Blue in Serratia sp Strain DRY5 Is Catalyzed by a Novel Molybdenum-Reducing Enzyme

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dc.contributor.authorShukor, MYen_US
dc.contributor.authorHalmi, MIEen_US
dc.contributor.authorRahman, MFAen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSyed, MAen_US
dc.date.accessioned2024-05-29T02:57:17Z
dc.date.available2024-05-29T02:57:17Z
dc.date.issued2014
dc.description.abstractThe first purification of the Mo-reducing enzyme from Serratia sp. strain DRY5 that is responsible for molybdenum reduction to molybdenum blue in the bacterium is reported. The monomeric enzyme has an apparent molecular weight of 105 kDalton. The isoelectric point of this enzyme was 7.55. The enzyme has an optimum pH of 6.0 and maximum activity between 25 and 35 degrees C. The Mo-reducing enzyme was extremely sensitive to temperatures above 50 degrees C (between 54 and 70 degrees C). A plot of initial rates against substrate concentrations at 15mM 12-MP registered a V-max for NADH at 12.0 nmole Mo blue/min/mg protein. The apparent k(m) for NADH was 0.79mM. At 5mM NADH, the apparent V-max and apparent k(m) values for 12-MP of 12.05 nmole/min/mg protein and 3.87mM, respectively, were obtained. The catalytic efficiency (k(cat)/k(m)) of the Mo-reducing enzyme was 5.47M(-1) s(-1). The purification of this enzyme could probably help to solve the phenomenon of molybdenum reduction to molybdenum blue first reported in 1896 and would be useful for the understanding of the underlying mechanism in molybdenum bioremediation involving bioreduction.
dc.identifier.doi10.1155/2014/853084
dc.identifier.issn2314-6133
dc.identifier.scopusWOS:000332932300001
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11631
dc.languageEnglish
dc.language.isoen_US
dc.publisherHindawi Ltden_US
dc.relation.ispartofBiomed Research International
dc.sourceWeb Of Science (ISI)
dc.titleMolybdenum Reduction to Molybdenum Blue in Serratia sp Strain DRY5 Is Catalyzed by a Novel Molybdenum-Reducing Enzyme
dc.typeArticleen_US
dspace.entity.typePublication

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