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Effect of bacterial growth period on the sensitivity of the MTT assay for silver

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dc.Chemicals/CASsilver, 7440-22-4
dc.citedby1
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorHalmi M.I.E.en_US
dc.contributor.authorAhmad F.en_US
dc.contributor.authorHashim A.K.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorSyed M.A.en_US
dc.contributor.authorShukor M.Y.en_US
dc.date.accessioned2024-05-29T02:01:33Z
dc.date.available2024-05-29T02:01:33Z
dc.date.issued2014
dc.description.abstractRespiratory activity inhibition by toxic compounds in bacteria and yeast has been used to detect toxic compounds in the environment. Often the age of culture contributes towards the sensitivity of detection. In the present work, the effect of growth period on the sensitivity of an inhibitive assay for heavy metals using bacterial respiratory assay system based on the reduction of the water soluble tetrazolium dye MTT is reported. A silver-sensitive isolate was discovered to exhibit different sensitivities towards silver at different growth periods. An exponential decay model adequately described the inhibition due to silver. Analysis using ANOVA with post-hoc Tukey's test showed that the IC 50 obtained by strain DRYS8 grown at the 12 hr-period in nutrient broth at 287deg;C gave the lowest value compared to other growth periods. This study highlights the importance of taking into accounts growth conditions and age of culture in developing cellular-based bioassays. � Triveni Enterprises, Lucknow (India).en_US
dc.description.natureFinalen_US
dc.identifier.CODENJEBID
dc.identifier.epage355
dc.identifier.issn2548704
dc.identifier.issue2
dc.identifier.pmid24665761
dc.identifier.scopus2-s2.0-84898681782
dc.identifier.spage353
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84898681782&partnerID=40&md5=c0bf294336af29050ee27c5fc87383cb
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10173
dc.identifier.volume35
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.ispartofJournal of Environmental Biology
dc.sourceScopus
dc.subjectBacteriumen_US
dc.subjectGrowth perioden_US
dc.subjectMTT assayen_US
dc.subjectSilveren_US
dc.titleEffect of bacterial growth period on the sensitivity of the MTT assay for silveren_US
dc.title.alternativeJ. Environ. Biol.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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