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Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation

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dc.contributor.authorEzeilo, Uchenna R.en_US
dc.contributor.authorWahab, Roswanira Abdulen_US
dc.contributor.authorMahat, Naji Arafaten_US
dc.date.accessioned2024-05-29T03:25:11Z
dc.date.available2024-05-29T03:25:11Z
dc.date.issued2020
dc.description.abstractIncreasing energy demands call for sustainable alternative sources. Solid state fermentation (SSF) of raw oil palm frond leaves (OPFL) as the substrate to produce extracellular cellulases and xylanase by a novel Rhizopus oryzae UC2 (GenBank accession no. MF767597) was optimized. Optimum SSF conditions (30 degrees C, 40% moisture content, 2.0 x 10(8) spores/g inoculum size) yielded the maximum carboxymethyl cellulase (CMCase) (94.68 U/g), filter paperase (FPase) (25.46 U/g), beta-glucosidase (145.47 U/g) and xylanase (213.99 U/g) activities, showing a broad pH range of between 6.0 and 12.0. Proteome analysis of crude enzyme cocktail revealed three beta-glucosidases, as well as one endo-beta-1,4-glucanase, exoglanase and endo-beta-1,4-xylanase each. Activities of the enzyme complex were maximal at an acidic pH and temperature that ranged between pH 3.0-5.0 and 50-60 degrees C, respectively. In situ hydrolysis of OPFL released various concentrations of sugars viz. glucose (26.74 mg/g), xylose (1.44 mg/g), fructose (50.8 mg/g) and cellobiose (58.31 mg/g). Moreover, CMCase, FPase, beta-glucosidase and xylanase exhibited half-lives of 5.13, 1.48, 18.81, 9.23 h when incubated at 60 degrees C, respectively. Thus, the desirable qualities of R. oryzae UC2 seen here supported its prospective biocatalytic role for timely and safe production of digestible carbohydrates from agro-industrial biomass for the subsequent biotransformation into biofuel. (C) 2019 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.renene.2019.11.149
dc.identifier.epage1312
dc.identifier.issn0960-1481
dc.identifier.scopusWOS:000540378500105
dc.identifier.spage1301
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11898
dc.identifier.volume156
dc.languageEnglish
dc.language.isoen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofRenewable Energy
dc.subjectCellulaseen_US
dc.subjectXylanaseen_US
dc.subjectRhizopus oryzaeen_US
dc.subjectSSFen_US
dc.subjectOptimizationen_US
dc.subjectOil palm leavesen_US
dc.titleOptimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 using raw oil palm frond leaves as substrate under solid state fermentation
dc.typeArticleen_US
dspace.entity.typePublication

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