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Metabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03

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dc.contributor.authorLiyana Amalina Adnanen_US
dc.contributor.authorPalanivel Sathishkumaren_US
dc.contributor.authorAbdull Rahim Mohd Yusoffen_US
dc.contributor.authorTony Hadibarataen_US
dc.date.accessioned2024-05-28T03:53:23Z
dc.date.available2024-05-28T03:53:23Z
dc.date.issued2015
dc.date.submitted29/10/2019
dc.description.abstractIn this study, fast growing ascomycete fungus Trichoderma atroviride F03 was explored to biodegrade bis-azo dye, Reactive Black 5 (RB5). The maximum RB5 biodegradation (91.1%) was achieved in the culture medium supplemented with an appropriate carbon source (glucose, 20 g l−1), and nitrogen source (yeast extract, 20 g l−1) at pH 5 and 27 °C. The laccase produced by T. atroviride F03 was involved in the RB5 biodegradation processes. The metabolites such as (I) 1,2,4-trimethylbenzene, (II) 2,4-ditertbutylphenol, and (III) benzoic acid-TMS) were identified as the biodegradation products of RB5 using gas chromatography-mass spectrometry (GC–MS). The presence of these metabolites suggested that RB5 biodegradation was initiated by the cleavage of azo bond forming naphthalene-1,2,8-triol and sulphuric acid mono-[2-(toluene-4-sulfonyl)-ethyl] ester. The sulphuric acid mono-[2-(toluene-4-sulfonyl)-ethyl] ester was further desulphonated to 1,2,4-trimethylbenzene. Then, the oxygenated ring of C1 and C2 naphthalene-1,2,8-triol was cleaved to 2-(2-carboxy-ethyl)-6-hydroxy-benzoic acid. The degradation of 2-(2-carboxy-ethyl)-6-hydroxy-benzoic acid could be proceeded with two pathways: (i) decarboxylation and methylation to form 2,4-ditertbutylphenol and (ii) decarboxylation mechanism that induced the formation of benzoic acid-TMS. Finally, this study proved that T. atroviride F03 might be a good candidate in treating textile effluent containing azo dye as this treatment does not generating aromatic amines. Keywords Azo dyeBiodegradation, Laccase, Metabolic pathway, Reactive black 5, Trichoderma atroviride F03en_US
dc.identifier.citationLiyana Amalina Adnan, Palanivel Sathishkumar, Abdull Rahim Mohd Yusoff, Tony Hadibarata, Metabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03, International Biodeterioration & Biodegradation,Volume 104,2015,Pages 274-282,ISSN 0964-8305, https://doi.org/10.1016/j.ibiod.2015.05.019.en_US
dc.identifier.doi10.1016/j.ibiod.2015.05.019
dc.identifier.epage282
dc.identifier.issn0964-8305
dc.identifier.other2236-4
dc.identifier.spage274
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0964830515300159?via%3Dihub
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/5197
dc.identifier.volume104
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_US
dc.subjectAzo dyeBiodegradation,en_US
dc.subjectLaccase,en_US
dc.subjectMetabolic pathway,en_US
dc.subjectReactive black 5,en_US
dc.subjectTrichoderma atroviride F03en_US
dc.titleMetabolites characterisation of laccase mediated Reactive Black 5 biodegradation by fast growing ascomycete fungus Trichoderma atroviride F03en_US
dc.typeArticleen_US
dspace.entity.typePublication

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