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The assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ions

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dc.Chemicals/CASarsenic, 7440-38-2; butyrylthiocholine, 1866-16-6, 4555-00-4; cadmium, 22537-48-0, 7440-43-9; cholinesterase, 9001-08-5; chromium, 16065-83-1, 7440-47-3, 14092-98-9; copper, 15158-11-9, 7440-50-8; lead, 7439-92-1, 13966-28-4; mercury, 14302-87-5, 7439-97-6; procainamide, 51-06-9, 614-39-1; silver, 7440-22-4; zinc, 7440-66-6, 14378-32-6; Cholinesterases; Metals, Heavy
dc.citedby15
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsAsia Metropolitan University
dc.contributor.authorSabullah M.K.en_US
dc.contributor.authorSulaiman M.R.en_US
dc.contributor.authorShukor M.Y.A.en_US
dc.contributor.authorSyed M.A.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorKhalid A.en_US
dc.contributor.authorAhmad S.A.en_US
dc.date.accessioned2024-05-28T08:24:42Z
dc.date.available2024-05-28T08:24:42Z
dc.date.issued2014
dc.description.abstractCrude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 μmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and -25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions.en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo571094
dc.identifier.doi10.1155/2014/571094
dc.identifier.issn23566140
dc.identifier.pmid25401148
dc.identifier.scopus2-s2.0-84911367743
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84911367743&doi=10.1155%2f2014%2f571094&partnerID=40&md5=2cabcca986e5651bc46209f1beaba391
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8547
dc.identifier.volume2014
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherHindawi Publishing Corporationen_US
dc.relation.ispartofOpen Accessen_US
dc.relation.ispartofScientific World Journal
dc.sourceScopus
dc.titleThe assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ionsen_US
dc.title.alternativeSci. World J.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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