Publication:
Optimized Sample Preparation Technique For Visualization Of The Adherence Of Lactobacillaceae Sp. To Human Colorectal Adenocarcinoma Cell Line Ht-29 By Field Emission Scanning Electron Microscope

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dc.contributor.authorIlyanie H.Y
dc.contributor.authorIda Muryany M.Y.
dc.contributor.authorHuda-Faujan N.
dc.date.accessioned2024-06-16T06:35:58Z
dc.date.available2024-06-16T06:35:58Z
dc.date.issued2023
dc.date.submitted2024-1-23
dc.descriptionMalaysian Journal of Biochemistry & Molecular Biology, Volume 1(Special issue), Page (16–22)
dc.description.abstractThe objective of this study was to compare and determine the optimal sample preparation techniques to observe the adherence of probiotic bacteria, Lactobacillaceae sp. to human colorectal adenocarcinoma cell line HT-29. Different fixation time and coating thickness were compared to evaluate the integrity and quality of image observed using field emission scanning electron microscope (FESEM). Based on the qualitative assessments, the one-hour fixation was proposed as a promising fixation period as it is better than overnight fixation in terms of showing clearer parts and the adhesive features on the surface of both bacteria and HT-29 cells. Platinum sputter-coating step is recommended at shorter time to increase conductivity of sample and reduce electron beam damage. Thicker platinum coat could obscure fine structural visualization although it helps in eliminating charging during the imaging process. This finding provides an important sample preparation optimization to enable better structural integrity and quality of image captured using FESEM.
dc.identifier.citationIlyanie H.Y., Ida Muryany M.Y., & Huda-Faujan N.3. (2023). Optimized Sample Preparation Technique For Visualization Of The Adherence Of Lactobacillaceae Sp. To Human Colorectal Adenocarcinoma Cell Line Ht-29 By Field Emission Scanning Electron Microscope. Malaysian Journal of Biochemistry & Molecular Biology, 1(special issue), 16–22.
dc.identifier.epage22
dc.identifier.issn1511-2616
dc.identifier.issueSpecial Is
dc.identifier.other312-33
dc.identifier.spage16
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/19927
dc.identifier.urihttps://1f169227-0328-4af2-99bc-24934f131be8.filesusr.com/ugd/66925b_8dab673e57e54c46b1181b158e911329.pdf
dc.identifier.volume1
dc.language.isoen_US
dc.publisherThe Malaysian Society for Biochemistry & Molecular
dc.relation.ispartofMalaysian Journal of Biochemistry and Molecular Biology
dc.relation.issn2600-9005
dc.relation.journalMalaysian Journal of Biochemistry & Molecular Biology
dc.subjectCell adherence
dc.subjectFESEM
dc.subjectLactobacillaceae
dc.subjectHT-29
dc.subjectFixation
dc.titleOptimized Sample Preparation Technique For Visualization Of The Adherence Of Lactobacillaceae Sp. To Human Colorectal Adenocarcinoma Cell Line Ht-29 By Field Emission Scanning Electron Microscope
dc.typetext::journal::journal article
dspace.entity.typePublication
oaire.citation.endPage22
oaire.citation.issueSpecial Issue
oaire.citation.startPage16
oaire.citation.volume1
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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