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Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer

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dc.citedby12
dc.contributor.affiliationsFaculty of Engineering and Built Environment
dc.contributor.affiliationsUniversiti Malaysia Perlis (UniMAP)
dc.contributor.affiliationsUniversiti Teknologi PETRONAS (UTP)
dc.contributor.affiliationsDepartment of Oral Biology & Craniofacial Sciences
dc.contributor.affiliationsUniversity of Malaya (UM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversity of Madras
dc.contributor.authorCheen, OCen_US
dc.contributor.authorGopinath, SCBen_US
dc.contributor.authorPerumal, Ven_US
dc.contributor.authorArshad, MKMen_US
dc.contributor.authorLakshmipriya, Ten_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorHaarindraprasad, Ren_US
dc.contributor.authorRao, BSen_US
dc.contributor.authorHashim, Uen_US
dc.contributor.authorPandian, Ken_US
dc.date.accessioned2024-05-29T02:58:28Z
dc.date.available2024-05-29T02:58:28Z
dc.date.issued2017
dc.descriptionMicrochimica Acta volume 184, pages117–125 (2017)
dc.description.abstractThis article describes a sensitive impedimetric method for the determination of human blood coagulation factor IX protein (FIX) which is present in extremely low concentration in serum. An interdigitated electrode (IDE) whose surface was layered with zinc oxide was modified with two kinds of probes. One is an antibody, the other an aptamer against FIX. A comparative study between anti-FIX aptamer and anti-FIX antibody showed the aptamer to possess higher affinity for FIX. A sandwich aptamer assay was worked out by using the FIX-binding aptamer on the surface of the IDE. It has a detection limit as low as 10 pM which makes it 4 to 30-fold more sensitive than any other method reported for FIX. Moreover, to practice detection in clinical samples, FIX was detected from the human blood serum by spiking. In our perception, the sensitivity of the ZnO-modified IDE presented here makes it a promising tool for sensing clinically relevant analytes that are present in very low (sub-pM) concentrations.
dc.description.natureFinal
dc.identifier.CODENMIACA
dc.identifier.doi10.1007/s00604-016-2001-6
dc.identifier.epage125
dc.identifier.isbn1436-5073
dc.identifier.issn0026-3672
dc.identifier.issue1
dc.identifier.scopusWOS:000389353900010
dc.identifier.spage117
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84994210587&doi=10.1007%2fs00604-016-2001-6&partnerID=40&md5=b97c3806a1c010ecc29e67e22da1065c
dc.identifier.urihttps://link.springer.com/article/10.1007/s00604-016-2001-6
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11693
dc.identifier.volume184
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherSpringer-Verlag Wienen_US
dc.relation.ispartofMicrochimica Acta
dc.sourceWeb Of Science (ISI)
dc.subjectCoagulation
dc.subjectHemophilia
dc.subjectImpedance
dc.subjectResistance analysis
dc.subjectFactor IX
dc.subjectAptamer
dc.subjectMonoclonal antibody
dc.subjectClotting
dc.titleAptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayeren_US
dc.typeArticleen_US
dspace.entity.typePublication

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Aptamer-based Impedimetric Determination Of The Human Blood Clotting Factor Ix In Serum Using An Interdigitated Electrode Modified With A Zno Nanolayer
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