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A simplified system for simulation of Streptococcus mutans biofilm on healthy extracted human tooth as dental plaque model

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dc.FundingDetailsMinistry of Higher Education, Malaysia: FRGS/1/2014/SG06/USIM/02/1 Universiti Sains Islam Malaysia: USG-0216/FPG/30/16416
dc.FundingDetailsThe author would like to acknowledge the Ministry of Higher Education (MOHE), Malaysia for the Fundamental Research Grant Scheme (FRGS/1/2014/SG06/USIM/02/1) and Universiti Sains Islam Malaysia Research Grant Scheme (PPP/ USG-0216/FPG/30/16416) that has funded this study.
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorNadia Haliben_US
dc.contributor.authorNor Zaihana Abdul Rahmanen_US
dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorNabihah Roslanen_US
dc.contributor.authorNurhani Jauharen_US
dc.date.accessioned2024-05-28T08:30:41Z
dc.date.available2024-05-28T08:30:41Z
dc.date.issued2019
dc.description.abstractThe understanding and capability to simulate reproducible in vitro plaque model are important where this artificial plaque can be utilized as a tool in assessing the efficacy of tooth cleaning products. Previous studies were done on synthetic surfaces such as glass beads under a very strict, controlled environment. Thus, the aim of this study was to establish a simplified system to enable the formation of single species biofilm on the extracted natural tooth as plaque model. Healthy extracted natural tooth was incubated in broth mixture of Streptococcus mutans, containing 5% sucrose and stimulated saliva in a simplified system. The incubation was set at room temperature for 24, 48, and 72 hours. After 24 hours, the sticky deposit was formed on the surface of the tooth. The area of biofilm, stained with disclosing agent, expended from 19.05 ± 1.41 mm2 (24 hours) to 23.25 ± 0.35 mm2 (48 hours). However, no significant expansion of the plaque area was observed on 72 hours (23.83 ± 0.52 mm2). The biofilm was then observed under Infinite Focus Microscope (IFM) to measure the biofilm thickness. The preliminary attempt showed that plaque appeared as dark deposit with a certain thickness. Therefore, this simplified system is a reliable method for producing artificial plaque.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.7324/JAPS.2019.90215
dc.identifier.epage115
dc.identifier.issn22313354
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85064930869
dc.identifier.spage112
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85064930869&doi=10.7324%2fJAPS.2019.90215&partnerID=40&md5=eab1b45f78a3f2c05069877603a8d5bb
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8957
dc.identifier.volume9
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherOpen Science Publishers LLP Inc.en_US
dc.relation.ispartofOpen Accessen_US
dc.sourceScopus
dc.sourcetitleJournal of Applied Pharmaceutical Science
dc.subjectExtracted toothen_US
dc.subjectIn vitro biofilmen_US
dc.subjectPlaque simulationen_US
dc.subjectStreptococcus mutansen_US
dc.titleA simplified system for simulation of Streptococcus mutans biofilm on healthy extracted human tooth as dental plaque modelen_US
dc.title.alternativeJ. Appl. Pharm. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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