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Dillenia suffruticosa exhibited antioxidant and cytotoxic activity through induction of apoptosis and G2/M cell cycle arrest

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dc.Chemicals/CAS1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid), 28752-68-3; hexane, 110-54-3; methanol, 67-56-1; Antioxidants; Flavonoids; Phenols; Plant Extracts
dc.FundingDetailsUniversiti Putra Malaysia: 04-01-09-0714RU,�04-04-10-884FR
dc.FundingDetailsThis study was funded by Research University Grant Scheme (RUGS) of Universiti Putra Malaysia ( 04-01-09-0714RU ) and Fundamental Research Grant Scheme ( 04-04-10-884FR ).
dc.citedby19
dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Technologi MARA (UiTM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorArmania N.en_US
dc.contributor.authorYazan L.S.en_US
dc.contributor.authorMusa S.N.en_US
dc.contributor.authorIsmail I.S.en_US
dc.contributor.authorFoo J.B.en_US
dc.contributor.authorChan K.W.en_US
dc.contributor.authorNoreen H.en_US
dc.contributor.authorHisyam A.H.en_US
dc.contributor.authorZulfahmi S.en_US
dc.contributor.authorIsmail M.en_US
dc.date.accessioned2024-05-29T01:59:52Z
dc.date.available2024-05-29T01:59:52Z
dc.date.issued2013
dc.description.abstractEthnopharmacological relevance: Dillenia suffruticosa (Family: Dilleniaceae) locally known as Simpoh air has been reported to be used traditionally to treat cancerous growth. Therefore, the present study was attempted to investigate the antioxidant and cytotoxic properties of different parts (root, flower, fruit and leaf) of D. suffruticosa extracts. Methods and materials: In this study, direct solvent extraction (aqueous and methanol) from different parts of D. suffruticosa (root, flower, fruit and leaf) were carried out. Antioxidant activities of D. suffruticosa extract were determined by using DPPH, ABTS FRAP and ?-carotene bleaching assays. Cytotoxicity and cell cycle arrest of the active extract were determined using MTT assay and flow cytometer, respectively. Sequential solvent extraction (hexane, DCM, EtOAc, and MeOH) were also carried out in root of D. suffruticosa to further evaluate the antioxidant and cytotoxic activity of the different solvent extracts. Results: Methanol (MeOH) root extract showed the highest TPC, antioxidant and cytotoxic activities (especially towards HeLa) compared to others (P<0.05). Based on the results, sequential solvent extraction (hexane, DCM, EtOAc and MeOH) was carried out in the roots of D. suffruticosa. MeOH extract exhibited the highest antioxidant activities among others and significantly correlated (P<0.05) with TPC, suggesting the important contribution of phenolic compounds to its antioxidant activity. On the other hand, the DCM and EtOAc exhibited higher cytotoxic activity to selected cancer cells (HeLa, MCF-7, MDA-MB-231, A549 and HT29) compared to others. In short, there is no established correlation between antioxidant and cytotoxic activities of D. suffruticosa extracts indicating that an agent with high antioxidant activities will not necessarily possesses good cytotoxic activities in return. Qualitative phytochemical screening of D. suffruticosa extracts suggested the presence of saponins, triterpenes, sterols, and polyphenolic compounds which are believed to contribute to the cytotoxic activities. Conclusion: It is suggested that the cytotoxicity of the active extracts in HeLa was due to the induction of apoptosis and cell cycle arrest at G2/M. � 2013 Elsevier Ireland Ltd.en_US
dc.description.natureFinalen_US
dc.identifier.CODENJOETD
dc.identifier.doi10.1016/j.jep.2013.01.017
dc.identifier.epage535
dc.identifier.issn3788741
dc.identifier.issue2
dc.identifier.pmid23353897
dc.identifier.scopus2-s2.0-84875217640
dc.identifier.spage525
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84875217640&doi=10.1016%2fj.jep.2013.01.017&partnerID=40&md5=6498fdbc99840ddef6b15c6bf78b4ff4
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10089
dc.identifier.volume146
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofJournal of Ethnopharmacology
dc.sourceScopus
dc.subjectAntioxidanten_US
dc.subjectApoptosisen_US
dc.subjectCytotoxicen_US
dc.subjectDillenia suffruticosaen_US
dc.titleDillenia suffruticosa exhibited antioxidant and cytotoxic activity through induction of apoptosis and G2/M cell cycle arresten_US
dc.title.alternativeJ. Ethnopharmacol.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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