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A Simple Visual Ethanol Biosensor Based on Alcohol Oxidase Immobilized onto Polyaniline Film for Halal Verification of Fermented Beverage Samples

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dc.contributor.authorKuswandi, Ben_US
dc.contributor.authorIrmawati, Ten_US
dc.contributor.authorHidayat, MAen_US
dc.contributor.authorJayusen_US
dc.contributor.authorAhmad, Men_US
dc.date.accessioned2024-05-29T03:26:11Z
dc.date.available2024-05-29T03:26:11Z
dc.date.issued2014
dc.description.abstractA simple visual ethanol biosensor based on alcohol oxidase (AOX) immobilised onto polyaniline (PANI) film for halal verification of fermented beverage samples is described. This biosensor responds to ethanol via a colour change from green to blue, due to the enzymatic reaction of ethanol that produces acetaldehyde and hydrogen peroxide, when the latter oxidizes the PANI film. The procedure to obtain this biosensor consists of the immobilization of AOX onto PANI film by adsorption. For the immobilisation, an AOX solution is deposited on the PANI film and left at room temperature until dried (30 min). The biosensor was constructed as a dip stick for visual and simple use. The colour changes of the films have been scanned and analysed using image analysis software (i.e., ImageJ) to study the characteristics of the biosensor's response toward ethanol. The biosensor has a linear response in an ethanol concentration range of 0.01%-0.8%, with a correlation coefficient (r) of 0.996. The limit detection of the biosensor was 0.001%, with reproducibility (RSD) of 1.6% and a life time up to seven weeks when stored at 4 degrees C. The biosensor provides accurate results for ethanol determination in fermented drinks and was in good agreement with the standard method (gas chromatography) results. Thus, the biosensor could be used as a simple visual method for ethanol determination in fermented beverage samples that can be useful for Muslim community for halal verification.
dc.identifier.doi10.3390/s140202135
dc.identifier.epage2149
dc.identifier.issue2
dc.identifier.scopusWOS:000335887900013
dc.identifier.spage2135
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12082
dc.identifier.volume14
dc.languageEnglish
dc.language.isoen_US
dc.publisherMDPIen_US
dc.relation.ispartofSensors
dc.sourceWeb Of Science (ISI)
dc.subjecthalalen_US
dc.subjectpolyanilineen_US
dc.subjectalcohol oxidase ethanolen_US
dc.subjectbiosensoren_US
dc.subjectfermented beverageen_US
dc.titleA Simple Visual Ethanol Biosensor Based on Alcohol Oxidase Immobilized onto Polyaniline Film for Halal Verification of Fermented Beverage Samples
dc.typeArticleen_US
dspace.entity.typePublication

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