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Fluorescence bioanalytical method for urea determination based on water soluble ZnS quantum dots

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dc.contributor.authorSafitri, Een_US
dc.contributor.authorHeng, LYen_US
dc.contributor.authorAhmad, Men_US
dc.contributor.authorLing, TLen_US
dc.date.accessioned2024-05-29T03:25:41Z
dc.date.available2024-05-29T03:25:41Z
dc.date.issued2017
dc.description.abstractA new bioanalytical method for urea determination by using the immobilized urease on the highly luminescent ZnS quantum dots (QDs), which functioned as the pH fluorescent label has been developed. Bioconjugation of ZnS QDs to urease via amide bond was performed to obtain the ZnS QDs-urease bioconjugate. Upon enzymatic hydrolysis of urea by the immobilized urease, a pH change to a more alkaline condition has led to the deprotonation of ZnS QDs, and an increase in the fluorescence intensity can be observed. The fluorescence intensity of the urease conjugated ZnS QDs changed linearly with the urea concentrations between 4 x 10(-9) M and 4 x 10(-3) M (R-2 = 0.992) at pH 6 with a calibration sensitivity of 179.46 intensity/decade. No noticeable influence by the Ca2+, Mg2+, K+ and Na+ ions on the response of the fluorescent pH bioprobe. Urea determination in soil sample by using the urea bioprobe was in good agreement with the standard DMAB (p-dimethylaminobenzaldehyde) UV-vis spectrophotometric method. By employing the strategy of ZnS QDs-urease bioconjugation, the stable covalent link between the two substances has appeared to widen the dynamic range and lower the detection limit for urea compared to free enzyme and QDs in solution for bioassay of urea concentration. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.snb.2016.08.129
dc.identifier.epage769
dc.identifier.isbn0925-4005
dc.identifier.scopusWOS:000390622300090
dc.identifier.spage763
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12007
dc.identifier.volume240
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors And Actuators B-Chemical
dc.sourceWeb Of Science (ISI)
dc.subjectQuantum dotsen_US
dc.subjectZnSen_US
dc.subjectUrea biosensoren_US
dc.subjectUrease immobilizationen_US
dc.subjectFluorescent labelen_US
dc.titleFluorescence bioanalytical method for urea determination based on water soluble ZnS quantum dots
dc.typeArticleen_US
dspace.entity.typePublication

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