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Identification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patients

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dc.contributor.authorShahida Mohd-Saiden_US
dc.contributor.authorNur Fatimah Zaharah Salehuddinen_US
dc.contributor.authorSiti Nursyuhada Jafaren_US
dc.contributor.authorHaslinda Ramlien_US
dc.contributor.authorTuti Ningseh Mohd-Domen_US
dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.date.accessioned2024-05-28T06:41:59Z
dc.date.available2024-05-28T06:41:59Z
dc.date.issued2021
dc.descriptionVolume 21 Issue 1 Page (366-373)en_US
dc.description.abstractMolecular identiβication of bacteria are very important to develop databasegene bank in university. Databases of genes are very important to anno-tate a collection of nucleotide sequence and protein translation of oral bac-teria. This research was conducted to identify bacteria that present in theplaque sample among diabetic patients collected from the Department ofPeriodontology and Community Oral health Universiti Sains Islam Malaysia(USIM). A total of bacteria was successfully isolated on Blood Agar from peri-odontal patients’ plaques. Colony Forming Unit (CFU/mL) was calculated toestimate the number of viable bacteria in a sample after performed serialdilution. Gram stained was done for all isolated samples to differentiatebetween Gram positive and negative organisms. About 2.07 x 107CFU/mLof bacteria was successfully collected from periodontal plaques on diabetespatient. From the numbers, only 17 were selected to identify the species.Seven from the samples were Gram-positive bacteria, meanwhile ten sam-ples were identiβied as Gram-negative bacteria. PCR products were isolatedfrom the samples by using polymerase chain reaction analysis.Streptococ-cus mutans,Streptococcus gordonii,Streptococcus oralis,Streptococcus sangui-nis,Streptococcus agalactiae,Streptococcus lutetiensis,Streptococcus downei,,Aggregatibacter actinomycetemcomitans, Klebsiella pneumoniae,Enterobacterclocae,Salmonella enterica subs enterica,Enterobacter ludwigii, Enterobactermori,Enterobacter roggen kampii,Enterobacter cloacae,Enterobacter tabaciandKlebsiella pneumoniaewere identiβied from all samples via DNA sequenc-ing.en_US
dc.identifier.doi10.26452/ijrps.v12i1.4147
dc.identifier.epage373
dc.identifier.issn0975-7538
dc.identifier.issue1
dc.identifier.spage366
dc.identifier.urihttps://ijrps.com/index.php/home/article/view/252/916
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/7608
dc.identifier.volume12
dc.language.isoen_USen_US
dc.publisherJK Welfare & Pharmascope Foundationen_US
dc.relation.ispartofInternational Journal of Research in Pharmaceutical Sciencesen_US
dc.subjectPeriodontal disease,Diabetes,Bacteriaen_US
dc.titleIdentification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patientsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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