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Beta-mangostin demonstrates apoptogenesis in murine leukaemia (WEHI-3) cells in vitro and in vivo

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dc.Chemicals/CAScaspase 3, 169592-56-7; caspase 9, 180189-96-2; DNA, 9007-49-2; hoe 33342, 23491-52-3; lipocortin 5, 111237-10-6; protein bcl 2, 219306-68-0; vinblastine, 865-21-4; Antineoplastic Agents, Phytogenic; Caspase 3; Caspase 9; mangostin; Plant Extracts; Reactive Oxygen Species; xanthone; Xanthones
dc.citedby1
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversity of Malaya (UM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsSEGi University College
dc.contributor.affiliationsJazan University
dc.contributor.authorOmer F.A.A.en_US
dc.contributor.authorHashim N.M.en_US
dc.contributor.authorIbrahim M.Y.en_US
dc.contributor.authorAldoubi A.F.en_US
dc.contributor.authorHassandarvish P.en_US
dc.contributor.authorDehghan F.en_US
dc.contributor.authorNordin N.en_US
dc.contributor.authorKarimian H.en_US
dc.contributor.authorSalim L.Z.A.en_US
dc.contributor.authorAbdulla M.A.en_US
dc.contributor.authorAl-Jashamy K.en_US
dc.contributor.authorMohan S.en_US
dc.date.accessioned2024-05-28T08:34:25Z
dc.date.available2024-05-28T08:34:25Z
dc.date.issued2017
dc.description.abstractBackground: Beta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo. Methods: A WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects. Caspase 3 and 9 activities, ROS, western blot for Bcl2, and Bax were detected to study the mechanism of apoptosis. BALB/c mice injected with WEHI-3 cells were used to assess the apoptotic effect of BM in vivo. Results: BM suppressed the growth of WEHI-3 cells at an IC50value of 14 � 3 ?g/mL in 24 h. The ROS production was increased inside the cells in the treated doses. Both caspases (9 and 3) were activated in treating WEHI-3 cells at 24, 48 and 72 h. Different signs of apoptosis were detected, such as cell membrane blebbing, DNA segmentation and changes in the asymmetry of the cell membrane. Another action by which BM could inhibit WEHI-3 cells is to restrain the cell cycle at the G1/G0 phase. In the in vivo study, BM reduced the destructive effects of leukaemia on the spleen and liver by inducing apoptosis in leukaemic cells. Conclusion: BM exerts anti-leukaemic properties in vitro and in vivo. � 2017 The Author(s).en_US
dc.description.natureFinalen_US
dc.identifier.ArtNo366
dc.identifier.CODENBCAMC
dc.identifier.doi10.1186/s12906-017-1867-0
dc.identifier.issn14726882
dc.identifier.issue1
dc.identifier.pmid28716025
dc.identifier.scopus2-s2.0-85024115914
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85024115914&doi=10.1186%2fs12906-017-1867-0&partnerID=40&md5=0e73f10974312b85e842f356704c0f55
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9090
dc.identifier.volume17
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofOpen Accessen_US
dc.sourceScopus
dc.subjectApoptosisen_US
dc.subjectBALB/c miceen_US
dc.subjectBeta-mangostinen_US
dc.subjectWEHI-3 cell lineen_US
dc.titleBeta-mangostin demonstrates apoptogenesis in murine leukaemia (WEHI-3) cells in vitro and in vivoen_US
dc.title.alternativeBMC Complement. Altern. Med.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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