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In silico study of potential cross-kingdom plant microRNA based regulation in chronic myeloid leukemia

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dc.contributor.authorElias M.H.en_US
dc.contributor.authorNordin N.en_US
dc.contributor.authorHamid N.A.en_US
dc.date.accessioned2024-05-29T02:06:09Z
dc.date.available2024-05-29T02:06:09Z
dc.date.issued2020
dc.description.abstractBackground: Chronic Myeloid Leukaemia (CML) is associated with the BCR-ABL1 gene, which plays a central role in the pathogenesis of CML. Thus, it is crucial to suppress the expression of BCR-ABL1 in the treatment of CML. MicroRNA is known to be a gene expression regulator and is thus a good candidate for molecularly targeted therapy for CML. Objective: This study aims to identify the microRNAs from edible plants targeting the 3’ Untranslated Region (3’UTR) of BCR-ABL1. Methods: In this in silico analysis, the sequence of 3’UTR of BCR-ABL1 was obtained from Ensembl Genome Browser. PsRNATarget Analysis Server and MicroRNA Target Prediction (miRTar) Server were used to identify miRNAs that have binding conformity with 3’UTR of BCR-ABL1. The MiRBase database was used to validate the species of plants expressing the miRNAs. The RNAfold web server and RNA COMPOSER were used for secondary and tertiary structure prediction, respectively. Results: In silico analyses revealed that cpa-miR8154, csi-miR3952, gma-miR4414-5p, mdm-miR482c, osa-miR1858a and osa-miR1858b show binding conformity with strong molecular interaction towards 3’UTR region of BCR-ABL1. However, only cpa-miR-8154, osa-miR-1858a and osa-miR-1858b showed good target site accessibility. Conclusion: It is predicted that these microRNAs post-transcriptionally inhibit the BCR-ABL1 gene and thus could be a potential molecular targeted therapy for CML. However, further studies involving in vitro, in vivo and functional analyses need to be carried out to determine the ability of these miRNAs to form the basis for targeted therapy for CML. © 2020 Bentham Science Publishers.en_US
dc.identifier.doi10.2174/1875692118666200106113610
dc.identifier.epage132
dc.identifier.issn18756921
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85091646808
dc.identifier.spage125
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85091646808&doi=10.2174%2f1875692118666200106113610&partnerID=40&md5=91473fc30c711a7675eb44fba79ff266
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/10354
dc.identifier.volume17
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherBentham Science Publishersen_US
dc.relation.ispartofAfkaren_US
dc.sourceScopus
dc.subject3’ Untranslated Regionen_US
dc.subjectBCR-ABL1en_US
dc.subjectChronic myeloid leukemiaen_US
dc.subjectCross kingdomen_US
dc.subjectIn silicoen_US
dc.subjectMicroRNAen_US
dc.titleIn silico study of potential cross-kingdom plant microRNA based regulation in chronic myeloid leukemiaen_US
dc.typeArticleen_US
dspace.entity.typePublication

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