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Cholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metals

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dc.Chemicals/CASacetylthiocholine, 1797-69-9, 4468-05-7; ammonium sulfate, 7783-20-2; arsenic, 7440-38-2; cadmium, 22537-48-0, 7440-43-9; cholinesterase, 9001-08-5; chromium, 16065-83-1, 7440-47-3, 14092-98-9; iodide, 20461-54-5; mercury, 14302-87-5, 7439-97-6; silver, 7440-22-4
dc.citedby10
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsAsia Metropolitan University
dc.contributor.affiliationsUniversiti Malaysia Perlis (UniMAP)
dc.contributor.affiliationsUniversiti Teknologi MARA (UiTM)
dc.contributor.authorHayat N.M.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorShukor M.Y.en_US
dc.contributor.authorSabullah M.K.en_US
dc.contributor.authorSyed M.A.en_US
dc.contributor.authorKhalid A.en_US
dc.contributor.authorDahalan F.A.en_US
dc.contributor.authorKhalil K.A.en_US
dc.contributor.authorAhmad S.A.en_US
dc.date.accessioned2024-05-28T08:31:55Z
dc.date.available2024-05-28T08:31:55Z
dc.date.issued2015
dc.description.abstractHeavy metals are sometimes needed for nutrient uptake but only in low concentrations and the nervous system is the most susceptible to be affected. Cholinesterase (ChE) inhibition has been used extensively as a biomarker for heavy metals detection. In this study, the potential of ChE from Lates calcarifer brain as an alternative biosensor for heavy metals is evaluated. There are many Malaysian rivers such as Sungai Juru and Sungai Merbok that are greatly polluted by industrial effluents. Chronic exposure to heavy metals may cause nervous system disorders. Thus, a fast and simple biomonitoring technique will help in alerting government agencies and the public to such a threat. In this study, ChE from the brain of L. calcarifer (Asian seabass) was purified through ammonium sulphate precipitation and ion exchange chromatography. Enzyme recovery was 5.31% with a specific activity of 5.472 U mg-1. The Michaelis-Menten constant (Km) value was 0.3075 mM and a Vmax of 0.0304 ?mol min-1 mg-1. ChE from L. calcarifer brain showed higher affinity to acetylthiocholine iodide (ATC) compared to butyrylcholinesterase (BTC) and propionylcholinesterase iodide (PTC). Maximum activity of ChE was detected when the enzyme was assayed at the temperature of 20-30�C and incubated in Tris-HCl buffer pH 8.0. Silver (Ag), arsenic (As), cadmium (Cd), chromium (Cr) and mercury (Hg) showed more than 50% inhibition amongst the 10 types of heavy metals that have been tested for inhibition study. These results can be used to further develop an alternative way to detect heavy metals that are low cost and give faster results compared to existing biosensor kits.en_US
dc.description.natureFinalen_US
dc.identifier.epage381
dc.identifier.issn9742115
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84938580235
dc.identifier.spage376
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84938580235&partnerID=40&md5=d5afcdccdd41406b3d9e8552574abac3
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9001
dc.identifier.volume8
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherSPB Pharma Societyen_US
dc.relation.ispartofJournal of Chemical and Pharmaceutical Sciences
dc.sourceScopus
dc.subjectBiomarkeren_US
dc.subjectBiomonitoringen_US
dc.subjectCholinesteraseen_US
dc.subjectHeavy metalsen_US
dc.titleCholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metalsen_US
dc.title.alternativeJ. Chem. Pharm. Sci.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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