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Anticancer And Anti-inflammatory Activities Of Girinimbine Isolated From Murraya Koenigii

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dc.contributor.authorVenoos Imanen_US
dc.contributor.authorSyam Mohanen_US
dc.contributor.authorSiddig Ibrahim Abdelwahaben_US
dc.contributor.authorHamed Karimianen_US
dc.contributor.authorNoraziah Nordinen_US
dc.contributor.authorMehran Fadaeinasaben_US
dc.contributor.authorMohamad Ibrahim Noordinen_US
dc.contributor.authorSuzita Mohd Nooren_US
dc.date.accessioned2024-05-28T05:57:03Z
dc.date.available2024-05-28T05:57:03Z
dc.date.issued2017
dc.descriptionVolume :11en_US
dc.description.abstractTherapy that directly targets apoptosis and/or inflammation could be highly effective for the treatment of cancer. Murraya koenigii is an edible herb that has been traditionally used for cancer treatment as well as inflammation. Here, we describe that girinimbine, a carbazole alkaloid isolated from M. koenigii, induced apoptosis and inhibited inflammation in vitro as well as in vivo. Induction of apoptosis in human colon cancer cells (HT-29) by girinimbine revealed decreased cell viability in HT-29, whereas there was no cytotoxic effect on normal colon cells. Changes in mitochondrial membrane potential, nuclear condensation, cell permeability, and cytochrome c translocation in girinimbine-treated HT-29 cells demonstrated involvement of mitochondria in apoptosis. Early-phase apoptosis was shown in both acridine orange/propidium iodide and annexin V results. Girinimbine treatment also resulted in an induction of G0/G1 phase arrest which was further corroborated with the upregulation of two cyclin-dependent kinase proteins, p21 and p27. Girinimbine treatment activated apoptosis through the intrinsic pathway by activation of caspases 3 and 9 as well as cleaved caspases 3 and 9 which ended by triggering the execution pathway. Moreover, apoptosis was confirmed by downregulation of Bcl-2 and upregulation of Bax in girinimbine-treated cells. In addition, the key tumor suppressor protein, p53, was seen to be considerably upregulated upon girinimbine treatment. Induction of apoptosis by girinimbine was also evidenced in vivo in zebrafish embryos, with results demonstrating significant distribution of apoptotic cells in embryos after a 24-hour treatment period. Meanwhile, anti-inflammatory action was evidenced by the significant dose-dependent girinimbine inhibition of nitric oxide production in lipopolysaccharide/interferon-gamma-induced cells along with significant inhibition of nuclear factor-kappa B translocation from the cytoplasm to nucleus in stimulated RAW 264.7 cells. Girinimbine was also shown to have considerable antioxidant activity whereby 20 ?g/mL of girinimbine was equivalent to 82.17±1.88 µM of Trolox. In mice with carrageenan-induced peritonitis, oral pretreatment with girinimbine helped limit total leukocyte migration (mainly of neutrophils), and reduced pro-inflammatory cytokine levels (interleukin-1beta and tumor necrosis factor-alpha) in the peritoneal fluid. These findings strongly suggest that girinimbine could act as a chemopreventive and/or chemotherapeutic agent by inducing apoptosis while suppressing inflammation. There is a potential for girinimbine to be further investigated for its applicability in treating early stages of cancer.en_US
dc.identifier.doi10.2147/DDDT.S115135
dc.identifier.epage121
dc.identifier.issn1177-8881
dc.identifier.spage103
dc.identifier.urihttps://www.dovepress.com/anticancer-and-anti-inflammatory-activities-of-girinimbine-isolated-fr-peer-reviewed-fulltext-article-DDDT
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/6855
dc.identifier.volume11
dc.language.isoen_USen_US
dc.publisherDovepressen_US
dc.relation.ispartofDrug Design, Development and Therapyen_US
dc.subjectcarbazole alkaloid, apoptosis, inflammation, chemopreventive, chemotherapeuticen_US
dc.titleAnticancer And Anti-inflammatory Activities Of Girinimbine Isolated From Murraya Koenigiien_US
dc.typeArticleen_US
dspace.entity.typePublication

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