Publication:
Recovery of human DNA from canine teeth exposed to direct heating of 300 °c at varying durations for forensic identification

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dc.Conferencecode151647
dc.Conferencedate11 April 2019 through 12 April 2019
dc.Conferencename2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019
dc.FundingDetailsUniversiti Teknologi Malaysia: R.J130000.7854.4F990,�Q.J130000.2526.16H92
dc.FundingDetailsThe authors are thankful to Universiti Teknologi Malaysia for providing Research University Grant (Q.J130000.2526.16H92) and Fundamental Research Grant Scheme (R.J130000.7854.4F990) for conducting this research project. Thanks are also extend to DNA analysts at Forensic DNA Databank of Forensic Laboratory of Royal Malaysia Police.
dc.contributor.affiliationsCentre for Fiqh Forensics and Judiciary
dc.contributor.affiliationsUniversiti Teknologi Malaysia (UTM)
dc.contributor.affiliationsRoyal Malaysia Police
dc.contributor.affiliationsTudan Dental Clinic
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorMahat N.A.en_US
dc.contributor.authorDhevaseelan K.en_US
dc.contributor.authorSharif N.A.en_US
dc.contributor.authorKhan H.O.en_US
dc.contributor.authorAzman A.R.en_US
dc.contributor.authorLayang E.en_US
dc.contributor.authorIsmail S.A.en_US
dc.date.accessioned2024-05-28T08:28:30Z
dc.date.available2024-05-28T08:28:30Z
dc.date.issued2019
dc.description.abstractDental remains one of the most reliable forensic means for identification, especially when facial recognition and fingerprints are inconclusive. Even though previous studies reported the possibility of retrieving DNA from teeth exposed to 300 °C, its recovery at varying exposure durations at that temperature remains unexplored. Therefore, in order to acquire forensic significance, in this research, unclaimed canine teeth obtained from clinics and hospitals were divided into 0 min exposure group together with 4 other groups, and were exposed to 300 °C at varying durations. The dental pulps were extracted and subjected to DNA analyses viz. DNA extraction, quantitation, amplification and visualization, as well as profiling. Although the concentrations of DNA were found to be negatively and moderately correlated (P < 0.05, r = -0.669, r2 = 0.447) to the durations of exposure, complete human DNA profiles for the 0 min exposed sample and sample exposed to 300 °C for 5 mins alone were successfully obtained. This finding deserves consideration for whenever human DNA evidence from teeth exposed to such temperature and duration of exposure needs to be considered for human identification in forensic practical caseworks.en_US
dc.description.natureFinalen_US
dc.description.sponsorshipACS Malaysia Chapteren_US
dc.description.sponsorshipAdabien_US
dc.description.sponsorshipBersatu dan Setiaen_US
dc.description.sponsorshipBersatu Teguh Melakaen_US
dc.description.sponsorshipet al.en_US
dc.description.sponsorshipYayasan Feldaen_US
dc.editorMahat N.A.Wahab R.A.Huyop F.Z.Keyon A.S.A.Attan N.B.Chandren S.Gunam I.B.W.en_US
dc.identifier.ArtNo20004
dc.identifier.doi10.1063/1.5125508
dc.identifier.isbn9780740000000
dc.identifier.issn0094243X
dc.identifier.scopus2-s2.0-85072607241
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85072607241&doi=10.1063%2f1.5125508&partnerID=40&md5=18ae83a24cd392200d5456a5dc0a10ff
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/8857
dc.identifier.volume2155
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherAmerican Institute of Physics Inc.en_US
dc.relation.ispartofOpen Accessen_US
dc.sourceScopus
dc.sourcetitleAIP Conference Proceedings
dc.titleRecovery of human DNA from canine teeth exposed to direct heating of 300 °c at varying durations for forensic identificationen_US
dc.title.alternativeAIP Conf. Proc.en_US
dc.typeConference Paperen_US
dspace.entity.typePublication

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