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Potency of honey as antibacterial agent against multiple antibiotic resistant pathogens evaluated by different methods

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dc.citedby1
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.authorAween M.M.en_US
dc.contributor.authorHassan Z.en_US
dc.contributor.authorHuda-Faujan N.en_US
dc.contributor.authorEmdakim M.M.en_US
dc.contributor.authorMuhialdin B.J.en_US
dc.date.accessioned2024-05-28T08:46:37Z
dc.date.available2024-05-28T08:46:37Z
dc.date.issued2014
dc.description.abstractHoney is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dilution assay are methods commonly used to determine the antibacterial activity of honey. In this study, microtiter and microbial plate count were included to ascertain the potency of honey as antibacterial agent against multiple antibiotic resistant pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) with concentration of 0.2 g mL-1. Results found that well diffusion method tends to give higher inhibitory zone than disc diffusion method but there was no correlation among the bacteria was observed except for S. Typhimurium, E. coli (R = 0.310, 0.505 and 0.316, respectively). Nan photometer assay and microtiter plates assay showed comparable results with moderately strong correlation (R2 = 0.681 and 0.767, respectively) for S. aureus and S. typhimurium, but poor correlation was found for E. coli, B. subtilis and P. aeruginosa (R2 = 0.441, 0.308 and 0.383, respectively). Determining the number of survivors by plating on agar after nanophotometer assay or microtiter plate assay had confirmed the effectiveness of honey as antimicrobial agent against target bacteria; which confirmed that honey has the potency to inhibit pathogens even at low concentration. � 2014 M.M. Aween et al.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.3844/ofsp.9491
dc.identifier.epage1783
dc.identifier.issn15469239
dc.identifier.issue10
dc.identifier.scopus2-s2.0-84921925170
dc.identifier.spage1773
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84921925170&doi=10.3844%2fofsp.9491&partnerID=40&md5=9655f9aaa0492c7edd28199145499ad1
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9440
dc.identifier.volume11
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherScience Publicationsen_US
dc.relation.ispartofAmerican Journal of Applied Sciencesen_US
dc.sourceScopus
dc.subjectAntibacterial activity assayen_US
dc.subjectHoneyen_US
dc.subjectMultiple antibiotic resistant (MAR)en_US
dc.subjectPathogenic bacteriaen_US
dc.titlePotency of honey as antibacterial agent against multiple antibiotic resistant pathogens evaluated by different methodsen_US
dc.typeArticleen_US
dspace.entity.typePublication

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