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Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters

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dc.contributor.authorZaidan, UHen_US
dc.contributor.authorRahman, MBAen_US
dc.contributor.authorOthman, SSen_US
dc.contributor.authorBasri, Men_US
dc.contributor.authorAbdulmalek, Een_US
dc.contributor.authorAbd Rahman, RNZRen_US
dc.contributor.authorSalleh, Aen_US
dc.date.accessioned2024-05-29T03:26:18Z
dc.date.available2024-05-29T03:26:18Z
dc.date.issued2011
dc.description.abstractThe utilization of natural mica as a biocatalyst support in kinetic investigations is first described in this study. The formation of lactose caprate from lactose sugar and capric acid, using free lipase (free-CRL) and lipase immobilized on nanoporous mica (NER-CRL) as a biocatalyst, was evaluated through a kinetic study. The apparent kinetic parameters, K-m and V-max, were determined by means of the Michaelis-Menten kinetic model. The Ping-Pong Bi-Bi mechanism with single substrate inhibition was adopted as it best explains the experimental findings. The kinetic results show lower K-m values with NER-CRL than with free-CRL, indicating the higher affinity of NER-CRL towards both substrates at the maximum reaction velocity V-max,V-app > V-max). The kinetic parameters deduced from this model were used to simulate reaction rate data which were in close agreement with the experimental values.
dc.identifier.doi10.1271/bbb.110117
dc.identifier.epage1450
dc.identifier.issn0916-8451
dc.identifier.issue8
dc.identifier.scopusWOS:000294531200006
dc.identifier.spage1446
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12099
dc.identifier.volume75
dc.languageEnglish
dc.language.isoen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBioscience Biotechnology And Biochemistry
dc.sourceWeb Of Science (ISI)
dc.subjectlipase immobilizationen_US
dc.subjectmicaen_US
dc.subjectkinetic studyen_US
dc.subjectPing-Pong Bi-Bi modelen_US
dc.subjectsugar ester synthesisen_US
dc.titleKinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters
dc.typeArticleen_US
dspace.entity.typePublication

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