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Isolation and Characterization of Bacteriophage With Lytic Activity Against Carbapenem Resistance Strain of Klebsiella Pneumonia

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dc.contributor.authorAbeer Amen Baqer
dc.contributor.authorNorefrina Shafinaz Md Nor
dc.contributor.authorHuda Salman Alagely
dc.contributor.authorMustafa Musa
dc.contributor.authorSiti Noor Adnalizawati Adnan
dc.date.accessioned2024-06-16T06:36:23Z
dc.date.available2024-06-16T06:36:23Z
dc.date.issued2023
dc.date.submitted2024-2-15
dc.descriptionPol Merkur Lekarski
dc.description.abstractObjective: Aim: Klebsiella pneumonia has emerged as an increasingly important cause of community-acquired nosocomial infections and many of these strains are highly virulent and exhibit a strong propensity to spread. Infections cause by K. pneumonia produces carbapen¬emase (KPC) enzyme and can be difficult to treat since only a few antibiotics are effective against them. Bacteriophage targeting this strain can be an alternative treatment. Characterisation of bacteriophage is utmost important in assisting the application of bacteriophage in phage therapy. Patients and methods: Materials and methods: In the present study, the lytic bacteriophage, k3w7, isolated by the host Klebsiella pneumoniae kP2 was characterised using transmission electron microscope (TEM), plaque assay, and restriction digestive enzyme to investigate mor¬phology, host spectrum, bacteriophage life cycle and stability accordingly. Results: Results and conclusions: As shown by TEM, k3w7 was observed to have the characteristic of icosahedral heads 100 nm and contractile sheaths 120 nm suggesting it belongs to the family of myoviridae.The Investigation has done on the phage growth cycle showed a short latent period of 20 min and a burst size of approximately 220 plaque forming units per infected cell. Stability test showed the phage was stable over a wide range of pH and temperatures. According to restriction analysis, k3w7 had 50 -kb double-stranded DNA genome as well as the heterogeneous nature of genetic material. These findings suggest that K3W7 has a potential use in therapy against infections caused by K. pneumonia produces carbapenemase.
dc.identifier.doi10.36740/Merkur202301105
dc.identifier.epage41
dc.identifier.issn1426-9686
dc.identifier.issue1
dc.identifier.spage35
dc.identifier.urihttps://polskimerkuriuszlekarski.pl/wp-content/uploads/library/PolMerkurLek2023i1.pdf
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/19947
dc.identifier.volume51
dc.language.isoen_US
dc.publisherAluna Publishing
dc.relation.ispartofPolski Merkuriusz Lekarski
dc.relation.issn1426-9686
dc.relation.journalPol Merkur Lekarski .
dc.subjectBacteriophage
dc.subjectCarbapenemases
dc.subjectKlebsiella pneumonia
dc.subjectphage therapy.
dc.titleIsolation and Characterization of Bacteriophage With Lytic Activity Against Carbapenem Resistance Strain of Klebsiella Pneumonia
dc.typetext::journal::journal article::research article
dspace.entity.typePublication
oaire.citation.endPage41
oaire.citation.startPage35
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationUniversiti Sains Islam Malaysia

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