Publication:
Isolation and characterisation of phages targeting clinical Pseudomonas aeruginosa carrying virulence genes

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dc.contributor.affiliationsFaculty of Dentistry
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsQueen Elizabeth Hospital
dc.contributor.authorNur Afifah Binti Jamilen_US
dc.contributor.authorAbeer Ameen Baqeren_US
dc.contributor.authorSiti Noor Adnalizawati Adnanen_US
dc.contributor.authorAinulkhir Hussinen_US
dc.contributor.authorNorefrina Shafinaz Md. Noren_US
dc.date.accessioned2024-05-28T08:36:00Z
dc.date.available2024-05-28T08:36:00Z
dc.date.issued2018
dc.description.abstractAims: The aim of the study was to isolate and characterise bacteriophages specific to Pseudomonas aeruginosa carrying virulence genes. Methodology and results: Four clinical strains of P. aeruginosa CL1, CL2, CL3 and CL4 were obtained from Queen Elizabeth Hospital, Kota Kinabalu, Sabah. The bacterial strains were screened for virulence genes exoS, toxA and oprI and biofilm production. Six P. aeruginosa specific bacteriophages, namely PAtk1, PAtk2, PAtk3, PAtk4, PAtk5 and PAtk6, were isolated from Tasik Kejuruteraan, Universiti Kebangsaan Malaysia. These bacteriophages were screened for lytic spectrum against P. aeruginosa and two species of Enterobacteriaceae (Escherechia coli and Salmonella Typhi). PCR results showed that all strains possessed exoS, toxA and oprI genes except CL2 that lacked exoS. Nevertheless, it was CL2 that produced the highest biofilm density. Further, based on Transmission Electron Microscopy, PAtk15 and PAtk6 were classified into the family Myoviridae and Siphoviridae, respectively. Among all six isolated phages, only PAtk4 and PAtk6 showed the broadest lytic spectrum in which lytic activity was observed against all clinical P. aeruginosa strains. Conclusion, significance and impact of study: In this study we reported the isolation of six bacteriophages from Myoviridae and Siphoviridae that are specific to P. aeruginosa possessing exoS, toxA and oprI genes. Bacteriophages Patk4 and PAtk6 were able to infect all four strains of P. aeruginosa, making these phages potential agents in combating infections by the bacterium.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.21161/mjm.1461815
dc.identifier.epage569
dc.identifier.issn22317538
dc.identifier.issueSpecialissue6
dc.identifier.scopus2-s2.0-85061383739
dc.identifier.spage563
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061383739&doi=10.21161%2fmjm.1461815&partnerID=40&md5=af4f5195fdaf2784c78053461f64fc52
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9143
dc.identifier.volume14
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.relation.ispartofMalaysian Journal of Microbiologyen_US
dc.sourceScopus
dc.subjectExoSen_US
dc.subjectMyoviridaeen_US
dc.subjectOprIen_US
dc.subjectSiphoviridaeen_US
dc.subjectToxAen_US
dc.titleIsolation and characterisation of phages targeting clinical Pseudomonas aeruginosa carrying virulence genesen_US
dc.title.alternativeMalaysia. J. Microbiol.en_US
dc.typeArticleen_US
dspace.entity.typePublication

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