Publication:
Enhanced caffeine degradation by immobilised cells of Leifsonia sp strain SIU

Simple item page
dc.contributor.authorIbrahim, Sen_US
dc.contributor.authorShukor, MYen_US
dc.contributor.authorSyed, MAen_US
dc.contributor.authorJohari, WLWen_US
dc.contributor.authorShamaan, NAen_US
dc.contributor.authorSabullah, MKen_US
dc.contributor.authorAhmad, SAen_US
dc.date.accessioned2024-05-29T03:25:10Z
dc.date.available2024-05-29T03:25:10Z
dc.date.issued2016
dc.description.abstractIn a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine. The isolate was encapsulated in gellan gum and its ability to degrade caffeine was compared with the free cells. The optimal caffeine degradation was attained at a gellan gum concentration of 0.75% (w/v), a bead size of 4 mm diameter, and 250 beads per 100 mL of medium. At a caffeine concentration of 0.1 g/L, immobilised cells of the strain SIU degraded caffeine within 9 h, which is faster when compared to the case of free cells, in which it took 12 h to degrade. The immobilised cells degraded caffeine completely within 39 and 78 h at 0.5 and 1.0 g/L, while the free cells took 72 and 148 h at 0.5 and 1.0 g/L, respectively. At higher caffeine concentrations, immobilised cells exhibited a higher caffeine degradation rate. At concentrations of 1.5 and 2.0 g/L, caffeine-degrading activities of both immobilised and free cells were inhibited. The immobilised cells showed no loss in caffeine-degrading activity after being used repeatedly for nine 24-h cycles. The effect of heavy metals on immobilised cells was also tested. This study showed an increase in caffeine degradation efficiency when the cells were encapsulated in gellan gum.
dc.identifier.doi10.2323/jgam.62.18
dc.identifier.epage24
dc.identifier.isbn1349-8037
dc.identifier.issn0022-1260
dc.identifier.issue1
dc.identifier.scopusWOS:000374814700004
dc.identifier.spage18
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11890
dc.identifier.volume62
dc.languageEnglish
dc.language.isoen_US
dc.publisherMicrobiol Res Foundationen_US
dc.relation.ispartofJournal Of General And Applied Microbiology
dc.sourceWeb Of Science (ISI)
dc.subjectcaffeineen_US
dc.subjectdegradationen_US
dc.subjectgellan gumen_US
dc.subjectimmobilisationen_US
dc.subjectLeifsonia sp.en_US
dc.titleEnhanced caffeine degradation by immobilised cells of Leifsonia sp strain SIU
dc.typeArticleen_US
dspace.entity.typePublication

Files

Collections

Web of Science_WoS