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Nanogapped impedimetric immunosensor for the detection of 16 kDa heat shock protein against Mycobacterium tuberculosis

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dc.contributor.authorSubash C. B. Gopinathen_US
dc.contributor.authorVeeradasan Perumalen_US
dc.contributor.authorRamanujam Kumaresanen_US
dc.contributor.authorThangavel Lakshmipriyaen_US
dc.contributor.authorHaarindraprasad Rajintraprasaden_US
dc.contributor.authorBalakrishnan S. Raoen_US
dc.contributor.authorM. K. Md Arshaden_US
dc.contributor.authorYeng Chenen_US
dc.contributor.authorNorito Kotanien_US
dc.contributor.authorUda Hashimen_US
dc.date.accessioned2024-05-28T05:49:46Z
dc.date.available2024-05-28T05:49:46Z
dc.date.issued2016
dc.descriptionMicrochimica Acta volume 183, pages2697–2703 (2016) **Non open access articleen_US
dc.description.abstractThe 16 kDa heat shock protein (16 kDa HSP) against Mycobacterium tuberculosis (MT), expressed during the growth phase of MT, is a potential target in diagnostic tests for tuberculosis (TB). We describe here a method for impedimetric determination of the antigen by using a nanogapped dielectric surface consisting of a silver support coated with a thin finger-shaped coating made from zinc oxide and gold and patterned through a lift-off process. The electrode was characterized by scanning electron microscopy, field emission scanning electron microscopy, atomic force microscopy, and energy-dispersive X-ray spectroscopy. Surface chemical functionalization and immobilization of antibody against the 16 kDa HSP was evidenced by FTIR. In order to improve the detection limit, the antigen was conjugated to 10 nm gold nanoparticles. The resulting biosensor is capable of detecting the 16 kDa HSP in concentrations as low as 100 fM. The method covers a wide analytical range that extends from 100 fM to 1 nM.en_US
dc.identifier.citationGopinath, S.C.B., Perumal, V., Kumaresan, R. et al. Nanogapped impedimetric immunosensor for the detection of 16 kDa heat shock protein against Mycobacterium tuberculosis . Microchim Acta 183, 2697–2703 (2016). https://doi.org/10.1007/s00604-016-1911-7en_US
dc.identifier.doi10.1007/s00604-016-1911-7
dc.identifier.epage2703
dc.identifier.spage2697
dc.identifier.urihttps://link.springer.com/article/10.1007/s00604-016-1911-7#citeas
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/6619
dc.identifier.volume183
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMicrochimica Actaen_US
dc.subjectZnO, Gold Nanoparticle, Gold surface, Impedance, Immunosensor, Dielectric sensor, Nanolayer, Silicon wafer, Surface chemistry, Tubercle bacillusen_US
dc.titleNanogapped impedimetric immunosensor for the detection of 16 kDa heat shock protein against Mycobacterium tuberculosisen_US
dc.typeArticleen_US
dspace.entity.typePublication

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