Publication:
Organotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration

Simple item page
dc.contributor.authorFatimah, SSen_US
dc.contributor.authorChua, Ken_US
dc.contributor.authorTan, GCen_US
dc.contributor.authorAzmi, TIen_US
dc.contributor.authorTan, AEen_US
dc.contributor.authorRahman, HAen_US
dc.date.accessioned2024-05-29T03:26:25Z
dc.date.available2024-05-29T03:26:25Z
dc.date.issued2013
dc.description.abstractBackground aims. The aim of the present study was to evaluate the effects of air-liquid interface on the differentiation potential of human amnion epithelial cells (HAECs) to skin-like substitute in organotypic culture. Methods. HAECs at passage 1-2 were seeded onto a fibrin layer populated with human amnion mesenchymal cells to form the organotypic cultures. The organotypic HAECs were then cultured for 7, 14 and 21 d in two types of culture system: the submerged culture and the air-liquid interface culture. Cell morphogenesis was examined under the light and electron microscopes (transmission and scanning) and analyzed by immunohistochemistry. Results. Organotypic HAECs formed a single layer epithelium after 3 wk in submerged as well as air-liquid interface cultures. Ultrastructurally, desmosomes were observed in organotypic HAECs cultured in the air-liquid interface but not in the submerged culture. The presence of desmosomes marked the onset of early epidermal differentiation. Organotypic HAECs were positive against anti-CK18 and anti-CK14 in both the submerged and the air-liquid interface cultures. The co-expression of CK14 and CK18 suggested that differentiation of HAECs into skin may follow the process of embryonic skin development. However, weak expression of CK14 was observed after 2 and 3 wk of culture in air-liquid interface. CK10, involucrin, type IV collagen and laminin-5 expression was absent in organotypic HAECs. This observation reflects the initial process of embryonic epidermal differentiation and stratification. Conclusions. Results from the present study suggest that the air-liquid interface could stimulate early differentiation of organotypic HAECs to epidermal cells, with a potential use for skin regeneration.
dc.identifier.doi10.1016/j.jcyt.2013.05.003
dc.identifier.epage1041
dc.identifier.issn1465-3249
dc.identifier.issue8
dc.identifier.scopusWOS:000321993900014
dc.identifier.spage1030
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/12111
dc.identifier.volume15
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCytotherapy
dc.sourceWeb Of Science (ISI)
dc.subjectair-liquid interfaceen_US
dc.subjectepithelial stem cellsen_US
dc.subjectfibrinen_US
dc.subjecthuman amnion-derived stem cellsen_US
dc.subjectorganotypic cultureen_US
dc.subjectskin regenerationen_US
dc.titleOrganotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration
dc.typeArticleen_US
dspace.entity.typePublication

Files

Collections

Web of Science_WoS