Publication: Effect Of Different Extraction Solvents on Antioxidant Properties of Water Lily (Nymphaeceae Antares) Flower Petal And Stamen.
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Rynnye Lyan Resources
Abstract
Water lily (Nymphaeaceae antares) contains a vast array of phenolic compounds with
important natural antioxidative properties that could inhibit the adverse effects of the
reactive oxygen species produced in living things. These substances are complex in
composition which requires a suitable extraction medium to exploit the phenolic
compounds. This study was designed to determine the antioxidative properties in petal and
stamen of water lily extracted using different extraction solvents namely ethyl acetate and
60% methanol. Antioxidant properties of the samples were determined using 2,2-diphenyl
-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric thiocyanate (FTC) method,
thiobarbituric acid (TBA) test, total phenolic content (TPC), total flavonoid content (TFC)
and total tannin content (TTC) along with individual flavonoids content using High
Performance Liquid Chromatography (HPLC). The yield of extract was found to be the
highest in petals extracted with 60% methanol (44.55±1.63%) while stamen extracted with
ethyl acetate yielded the lowest (24.50±1.09%). In the DPPH inhibition method, petal
extracted with ethyl acetate obtained the highest scavenging activity (89.15±3.62%). FTC
analysis revealed that ethyl acetate extract of petal experienced the highest percentage
inhibition (76.70±6.48%) while in TBA analysis ethyl acetate extract of stamen exhibited
the highest percentage inhibition (70.78±1.39%). Flavonoids such as quercetin,
kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-coumaric acid and myricetin
were found to be present in ethyl acetate and 60% methanol extracts of water lilies stamen
and petal. The information from this study indicates that water lily petals and stamens may
be integrated as a good source of nutrients in the food industry, the pharmaceutical
industry. If the advantages of water lily are not used and integrated into everyday life for
health benefits, it will be a massive waste of natural resources.
Description
Keywords
Antioxidant activities, Nymphaea antares, Solvents extraction.
Citation
Mohd Zin, Z., Sanuri, A. Z. M., Bashah, N., Ibrahim, K., Yahya, H., & Zainol, M. K. (2021). Effect of different extraction solvents on antioxidant properties of water lily (nymphaeaceae antares) flower petal and stamen. Food Research, 5(3), 359-368. doi:10.26656/fr.2017.5(3).653