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Modeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology

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dc.contributor.authorArifin, Nen_US
dc.contributor.authorSoo-Peng, Ken_US
dc.contributor.authorLong, Ken_US
dc.contributor.authorTan, CPen_US
dc.contributor.authorYusoff, MSAen_US
dc.contributor.authorLai, OMen_US
dc.date.accessioned2024-05-29T03:25:37Z
dc.date.available2024-05-29T03:25:37Z
dc.date.issued2012
dc.description.abstractOptimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium- and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8-24 h), enzyme load (5-15 wt.%), and fatty acids/glycerol ratio (3:1-4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R (2) = 0.9507) and residual FFA (R (2) = 0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6-14.0 h for reaction time, 7.9-8.0 wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P > 0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production.
dc.identifier.doi10.1007/s11947-010-0325-5
dc.identifier.epage225
dc.identifier.issn1935-5130
dc.identifier.issue1
dc.identifier.scopusWOS:000299520700024
dc.identifier.spage216
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11994
dc.identifier.volume5
dc.languageEnglish
dc.language.isoen_US
dc.publisherSpringeren_US
dc.relation.ispartofFood And Bioprocess Technology
dc.sourceWeb Of Science (ISI)
dc.subjectEsterificationen_US
dc.subjectMedium- and long-chain triacyglycerols (MLCT)en_US
dc.subjectResponse surface methodology (RSM)en_US
dc.subjectReaction timeen_US
dc.subjectEnzyme loaden_US
dc.subjectFatty acids/glycerol molar ratioen_US
dc.subjectMLCT yielden_US
dc.subjectResidual FFAen_US
dc.titleModeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology
dc.typeArticleen_US
dspace.entity.typePublication

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