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Anti-Bacterial Activities of Melastoma Malabathricum Stem Bark Fractions against Streptococcus Mutans

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dc.contributor.authorRohazila Mohamad Hanafiahen_US
dc.contributor.authorSiti Aisyah Abd Ghafaren_US
dc.contributor.authorZurairah Ibrahimen_US
dc.contributor.authorWan Yaacob Wan Ahmaden_US
dc.contributor.authorWan Syaidatul Aqmaen_US
dc.contributor.authorNazlina Ibrahimen_US
dc.date.accessioned2024-05-30T02:08:14Z
dc.date.available2024-05-30T02:08:14Z
dc.date.issued2018
dc.description.abstractThe objective of this study is to isolate and identify the bioactive compounds that possess antibacterial activities from Melastoma malabathricum stem bark acetone extract (MMSBAE) against Streptococcus mutans. M. malabathricum is widely used in the Southeast Asia to treat many ailments. A total of 12 fractions was purified by vacuum liquid chromatography (VLC) and further analysed by TLC-bioautography to determine antibacterial activities. TLC-bioautography showed that fraction 9 possesses antibacterial activities against S. mutans. Identification of fraction 9 had been done by GCMS and revealed 21 compounds. Some of the compound were important as agent pharmaceutical such as α-amyrin, β-sitosterol, hexadecenoic acid, stearic acid and hexacosanoic acid. Crystal violet and glass surface assay were used to determine anti-biofilm and anti-adherence activity, respectively. The concentrations of fraction 9 that produce 50% reduction in anti-biofilm and anti-adherence activities were 5 mg/mL and 2.50 mg/mL, respectively. Scanning electron microscopy (SEM) was performed to visualize the effect of the fraction 9 on biofilm structure of S. mutans. SEM analysis showed lysed biofilm were found on treated cells. These results indicated that this fraction possesses a powerful anti-cariogenic potential against S. mutans.en_US
dc.identifier.citationHanafiah, R. M., Abd Ghafar, S. A., Ibrahim, Z., Wan Ahmad, W. Y., Wan Mohd Noor, W. S. A., & Ibrahim, N. (2018). Anti-Bacterial Activities of Melastoma Malabathricum Stem Bark Fractions against Streptococcus Mutans. Malaysian Journal of Science Health & Technology, 1. https://doi.org/10.33102/mjosht.v1i1.17en_US
dc.identifier.doi10.33102/mjosht.v1i1.17
dc.identifier.epage62
dc.identifier.issn2601-0003
dc.identifier.issue1
dc.identifier.spage58
dc.identifier.urihttp://mjosht.usim.edu.my/index.php/mjosht/article/view/17
dc.identifier.urihttps://mjosht.usim.edu.my/index.php/mjosht/article/view/17
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/15515
dc.identifier.volume1
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherUniversiti Sains Islam Malaysiaen_US
dc.relation.ispartofMalaysian Journal of Science, Health & Technologyen_US
dc.subjectMelastoma malabathricumen_US
dc.subjectStreptococcus mutansen_US
dc.titleAnti-Bacterial Activities of Melastoma Malabathricum Stem Bark Fractions against Streptococcus Mutansen_US
dc.typeArticleen_US
dspace.entity.typePublication

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