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An alternative bioassay using Anabas testudineus (Climbing perch) colinesterase for metal ions detection

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dc.FundingDetailsMinistry of Science, Innovation and Universities: FRGS/2/2013/SG05/UPM/02/16,�02-01-04-sf1473,�02-02-13-1256FR
dc.FundingDetailsThis project was supported by fund from The Ministry of Science, Technology and Innovation (MOSTI), Malaysia, under FRGS Grant no. 02-02-13-1256FR (FRGS/2/2013/SG05/UPM/02/16), Sciencefund (02-01-04-sf1473) and Pusat Sains Marin, UPM, Telok Kemang, Port Dickson, Malaysia.
dc.citedby9
dc.contributor.affiliationsFaculty of Medicine and Health Sciences
dc.contributor.affiliationsUniversiti Putra Malaysia (UPM)
dc.contributor.affiliationsUniversiti Malaysia Sabah (UMS)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.authorAhmad S.A.en_US
dc.contributor.authorWong Y.F.en_US
dc.contributor.authorShukor M.Y.en_US
dc.contributor.authorSabullah M.K.en_US
dc.contributor.authorYasid N.A.en_US
dc.contributor.authorHayat N.M.en_US
dc.contributor.authorShamaan N.A.en_US
dc.contributor.authorKhalid A.en_US
dc.contributor.authorSyed M.A.en_US
dc.date.accessioned2024-05-28T08:38:53Z
dc.date.available2024-05-28T08:38:53Z
dc.date.issued2016
dc.description.abstractClimbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided a recovery yield of 5.36% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM of butyrylthiocholine iodide (BTC) with pH 8.0 in Tris-HCl buffer at 40�C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (Vmax/Km). Protein analysis through Native-PAGE showed that ion exchange chromatography is an effective method to partially purify ChE. Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) shows the lowest inhibition effect (28.01%). All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions.en_US
dc.description.natureFinalen_US
dc.identifier.epage1452
dc.identifier.issn19854668
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84977590585
dc.identifier.spage1446
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84977590585&partnerID=40&md5=93da114337a9f289d50074ca946c60ce
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9227
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_USen_US
dc.publisherUniversiti Putra Malaysiaen_US
dc.relation.ispartofInternational Food Research Journalen_US
dc.sourceScopus
dc.subjectBioindicatoren_US
dc.subjectCholinesterase (ChE)en_US
dc.subjectInhibitionen_US
dc.subjectMetal ionsen_US
dc.titleAn alternative bioassay using Anabas testudineus (Climbing perch) colinesterase for metal ions detectionen_US
dc.typeArticleen_US
dspace.entity.typePublication

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