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Influence of N-limitation on malic enzyme isoforms and lipogenesis of Cunninghamella bainieri 2A1

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dc.citedby3
dc.contributor.affiliationsUniversiti Kebangsaan Malaysia (UKM)
dc.contributor.affiliationsUniversiti Sains Islam Malaysia (USIM)
dc.contributor.authorHamid A.A.en_US
dc.contributor.authorShuib S.en_US
dc.contributor.authorTaha E.M.en_US
dc.contributor.authorOmar O.en_US
dc.contributor.authorKhalil M.S.en_US
dc.contributor.authorKader A.J.A.en_US
dc.contributor.authorMohd Hafez Mohd Isaen_US
dc.date.accessioned2024-05-29T01:55:33Z
dc.date.available2024-05-29T01:55:33Z
dc.date.issued2014
dc.descriptionJurnal Teknologi (Sciences &Engineering) 67:1 (2014), 1–5en_US
dc.description.abstractThe influence of the presence of ammonium ions in growth culture on malic enzyme (ME) isoforms activity and lipogenesis in Cunninghamella bainieri 2A1 was investigated. The fungus was cultivated in a nitrogen-limiting medium for 120 h at 30°C under two conditions. One of the cultures was intermittently fed with ammonium tartrate to maintain the ammonium concentrations above 0.5 g/L. The second culture was performed without any feeding to allow N limitation, thus promoting lipid accumulation. Activity staining of ME isoforms was carried out for both cultures. The culture which was not intermittently fed with ammonium tartrate achieved a maximum lipid content of 35% (g/g biomass) at 48 h. This culture possessed five ME isoforms (A, B, C, D and E) with isoform E showing a parallel correlation to lipid accumulation profile. In contrast, intensity of bands representing isoform D decreased as lipid accumulated. No appreciable differences of all other isoforms were observed. However, the culture which was intermittently fed with ammonium tartrate, accumulated only up to 16% lipid (g/g biomass). All isoforms were present but with a more pronounced activity of isoform D and a lower activity of isoform E was observed. These findings support further evidence that isoform E is the key isoform for lipid synthesis in C. bainieri 2A1.en_US
dc.description.natureFinalen_US
dc.identifier.doi10.11113/jt.v67.1861
dc.identifier.epage5
dc.identifier.isiWOS:000218474700001
dc.identifier.issn0127-9696
dc.identifier.issue1
dc.identifier.scopus2-s2.0-84897606401
dc.identifier.spage1
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84897606401&doi=10.11113%2fjt.v67.1861&partnerID=40&md5=eb213277463901615a1c1b2435fd2402
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/9718
dc.identifier.volume67
dc.languageEnglish
dc.language.isoen_USen_US
dc.relation.ispartofJurnal Teknologi (Sciences and Engineering)en_US
dc.sourceScopus
dc.subjectLipid biosynthesisen_US
dc.subjectMalic enzyme isoformsen_US
dc.subjectNitrogen controlled-mediaen_US
dc.titleInfluence of N-limitation on malic enzyme isoforms and lipogenesis of Cunninghamella bainieri 2A1en_US
dc.typeArticleen_US
dspace.entity.typePublication

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