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Plagioneurin B, a potent isolated compound induces apoptotic signalling pathways and cell cycle arrest in ovarian cancer cells

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dc.contributor.authorNordin, Nen_US
dc.contributor.authorMajid, NAen_US
dc.contributor.authorOthman, Ren_US
dc.contributor.authorOmer, FAAen_US
dc.contributor.authorNasharuddin, MNAen_US
dc.contributor.authorHashim, NMen_US
dc.date.accessioned2024-05-29T03:25:12Z
dc.date.available2024-05-29T03:25:12Z
dc.date.issued2018
dc.description.abstractPlagioneurin B belongs to acetogenin group has well-established class of compounds. Acetogenin group has attracted worldwide attention in the past few years due their biological abilities as inhibitors for several types of tumour cells. Plagioneurin B was isolated via conventional chromatography and tested for thorough mechanistic apoptosis activity on human ovarian cancer cells (CAOV-3). Its structure was also docked at several possible targets using Autodock tools software. Our findings showed that plagioneurin B successfully inhibits the growth of CAOV-3 cells at IC50 of 0.62 A mu M. The existence of apoptotic bodies, cell membrane blebbing and chromatin condensation indicated the hallmark of apoptosis. Increase of Annexin V-FITC bound to phosphatidylserine confirmed the apoptosis induction in the cells. The apoptosis event was triggered through the extrinsic and intrinsic pathways via activation of caspases 8 and 9, respectively. Stimulation of caspase 3 and the presence of DNA ladder suggested downstream apoptotic signalling were initiated. Further confirmation of apoptosis was conducted at the molecular levels where up-regulation in Bax, as well as down-regulation of Bcl-2, Hsp-70 and survivin were observed. Plagioneurin B was also seen to arrest CAOV-3 cells cycle at the G2/M phase. Docking simulation of plagioneurin B with CD95 demonstrated that the high binding affinity and hydrogen bonds formation may explain the capability of plagioneurin B to trigger apoptosis. This study is therefore importance in finding the effective compound that may offer an alternative drug for ovarian cancer treatment.
dc.identifier.doi10.1007/s10495-018-1447-x
dc.identifier.epage169
dc.identifier.isbn1573-675X
dc.identifier.issn1360-8185
dc.identifier.issue2
dc.identifier.scopusWOS:000425541800005
dc.identifier.spage152
dc.identifier.urihttps://oarep.usim.edu.my/handle/123456789/11904
dc.identifier.volume23
dc.languageEnglish
dc.language.isoen_US
dc.publisherSpringeren_US
dc.relation.ispartofApoptosis
dc.sourceWeb Of Science (ISI)
dc.subjectPlagioneurin Ben_US
dc.subjectAcetogeninen_US
dc.subjectOvarian canceren_US
dc.subjectCAOV-3 cellsen_US
dc.subjectApoptosisen_US
dc.subjectPathwaysen_US
dc.titlePlagioneurin B, a potent isolated compound induces apoptotic signalling pathways and cell cycle arrest in ovarian cancer cells
dc.typeArticleen_US
dspace.entity.typePublication

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